Biochemistry (Looseleaf)
Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
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Chapter 4, Problem 21P
Interpretation Introduction

Interpretation:

The definition of retrovirus along with its flow of information as compared to an infected cell should be explained.

Concept introduction:

The flow of genetic information depends on the genetic code that is DNA or RNA.

Retrovirus is a type of virus which has RNA as a genetic medium. It uses reverse transcriptase to convert RNA into DNA which is integrated into a host cell.

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9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and 14.2 kDa, a) Figure 4: indicate where each of the measurements were taken and label as in II.6. figure 2 above. b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f. values for the MW standards, plot the relative mobility versus the log of the mass for the standards, and use the best fit straight line to determine the molecular weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be scaled up appropriately if your gel did not develop properly) dye MW Whey Peak 1 Peak 2
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