Human Anatomy
6th Edition
ISBN: 9781260399820
Author: SALADIN, Kenneth
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 4, Problem 10TYR
For the first 8 weeks of gestation, a conceptus is nourished mainly by
- the placenta.
- amniotic fluid.
- colostrum.
- decidual cells.
- yolk cytoplasm.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 4 Solutions
Human Anatomy
Ch. 4.1 - Prob. 1BYGOCh. 4.1 - Prob. 2BYGOCh. 4.1 - Explain the functions of the sperm’s acrosome and...Ch. 4.1 - Describe two ways a fertilized egg prevents the...Ch. 4.2 - List the four primary tissue types of the adult...Ch. 4.2 - Oligohydramnios17 is an abnormally low volume of...Ch. 4.2 - Prob. 5BYGOCh. 4.2 - Prob. 6BYGOCh. 4.2 - Prob. 7BYGOCh. 4.2 - Prob. 8BYGO
Ch. 4.2 - Prob. 9BYGOCh. 4.2 - Prob. 10BYGOCh. 4.2 - Define and describe the neural tube, primitive...Ch. 4.3 - Prob. 1AWYKCh. 4.3 - Prob. 12BYGOCh. 4.3 - Prob. 13BYGOCh. 4.3 - Why is a baby more likely to be born with...Ch. 4 - Prob. 4.1.1AYLOCh. 4 - Prob. 4.1.2AYLOCh. 4 - Prob. 4.1.3AYLOCh. 4 - Prob. 4.1.4AYLOCh. 4 - Prob. 4.1.5AYLOCh. 4 - Prob. 4.1.6AYLOCh. 4 - Prob. 4.1.7AYLOCh. 4 - The duration of pregnancy and how the date of...Ch. 4 - Prob. 4.2.2AYLOCh. 4 - Prob. 4.2.3AYLOCh. 4 - Prob. 4.2.4AYLOCh. 4 - Prob. 4.2.5AYLOCh. 4 - Prob. 4.2.6AYLOCh. 4 - Prob. 4.2.7AYLOCh. 4 - Prob. 4.2.8AYLOCh. 4 - Prob. 4.2.9AYLOCh. 4 - The major changes that occur during the embryonic...Ch. 4 - Prob. 4.2.11AYLOCh. 4 - How the coelom forms, how it divides into the...Ch. 4 - The meaning of organogenesis, and some tissues and...Ch. 4 - Prob. 4.2.14AYLOCh. 4 - Prob. 4.2.15AYLOCh. 4 - Prob. 4.2.16AYLOCh. 4 - Prob. 4.2.17AYLOCh. 4 - Prob. 4.2.18AYLOCh. 4 - Prob. 4.2.19AYLOCh. 4 - The three modes in which the conceptus is...Ch. 4 - The development and mature structure of the...Ch. 4 - The relationship of the placenta to the umbilical...Ch. 4 - Prob. 4.2.23AYLOCh. 4 - The time at which the individual is considered to...Ch. 4 - Prob. 4.2.25AYLOCh. 4 - Prob. 4.3.1AYLOCh. 4 - Prob. 4.3.2AYLOCh. 4 - Prob. 4.3.3AYLOCh. 4 - Prob. 4.3.4AYLOCh. 4 - Prob. 4.3.5AYLOCh. 4 - Prob. 4.3.6AYLOCh. 4 - Prob. 4.3.7AYLOCh. 4 - When a conceptus arrives in the uterus, it is at...Ch. 4 - The entry of a sperm nucleus into an egg must be...Ch. 4 - Prob. 3TYRCh. 4 - Prob. 4TYRCh. 4 - Prob. 5TYRCh. 4 - Prob. 6TYRCh. 4 - Prob. 7TYRCh. 4 - Prob. 8TYRCh. 4 - Prob. 9TYRCh. 4 - For the first 8 weeks of gestation, a conceptus is...Ch. 4 - Viruses and chemicals that cause congenital...Ch. 4 - Prob. 12TYRCh. 4 - Prob. 13TYRCh. 4 - Prob. 14TYRCh. 4 - Prob. 15TYRCh. 4 - The enzymes with which a sperm penetrates an egg...Ch. 4 - Prob. 17TYRCh. 4 - Prob. 18TYRCh. 4 - Prob. 19TYRCh. 4 - Prob. 20TYRCh. 4 - Prob. 1BYMVCh. 4 - Prob. 2BYMVCh. 4 - Prob. 3BYMVCh. 4 - Prob. 4BYMVCh. 4 - Prob. 5BYMVCh. 4 - Prob. 6BYMVCh. 4 - Prob. 7BYMVCh. 4 - Prob. 8BYMVCh. 4 - Prob. 9BYMVCh. 4 - Prob. 10BYMVCh. 4 - Prob. 1WWWTSCh. 4 - Prob. 2WWWTSCh. 4 - Prob. 3WWWTSCh. 4 - Prob. 4WWWTSCh. 4 - Prob. 5WWWTSCh. 4 - Prob. 6WWWTSCh. 4 - Prob. 7WWWTSCh. 4 - Prob. 8WWWTSCh. 4 - Prob. 9WWWTSCh. 4 - Briefly explain why each of the following...Ch. 4 - Only one sperm is needed to fertilize an egg, yet...Ch. 4 - Prob. 2TYCCh. 4 - Prob. 3TYCCh. 4 - Prob. 4TYCCh. 4 - Prob. 5TYC
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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