1 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781265486297
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 37.5, Problem 2LO
Summary Introduction
To explain: That how popular trees have been used for phytoremediation.
Introduction: The plants are mainly multicellular, predominantly photosynthetic eukaryotes of the kingdom Plantae. The trees are perennial plants that have an elongated trunk or stem, supporting branches and leaves in most species.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 37 Solutions
1 SEM ACC W/RAVEN CARDED
Ch. 37.1 - Prob. 1LOCh. 37.1 - Prob. 2LOCh. 37.1 - Prob. 3LOCh. 37.2 - Prob. 1LOCh. 37.2 - Prob. 2LOCh. 37.2 - Describe the goal of food fortification research.Ch. 37.3 - Prob. 1LOCh. 37.3 - Prob. 2LOCh. 37.3 - Prob. 3LOCh. 37.4 - Prob. 1LO
Ch. 37.4 - Explain the main effect on herbivores of a higher...Ch. 37.4 - Prob. 3LOCh. 37.5 - Prob. 1LOCh. 37.5 - Prob. 2LOCh. 37.5 - Prob. 3LOCh. 37 - Prob. 1IQCh. 37 - Prob. 2IQCh. 37 - Which of the following is NOT found in topsoil? a....Ch. 37 - Prob. 2UCh. 37 - What proportion of the soil volume is occupied by...Ch. 37 - Which of the following is a micronutrient? a....Ch. 37 - Prob. 5UCh. 37 - Prob. 6UCh. 37 - a C4 plant, the Calvin cycle occurs in a. the...Ch. 37 - One potential problem with using poplars to remove...Ch. 37 - Prob. 1ACh. 37 - If you wanted to conduct an experiment to...Ch. 37 - Which of the following would decrease nitrogen...Ch. 37 - Which of the following might you do to increase...Ch. 37 - Prob. 5ACh. 37 - Prob. 1SCh. 37 - Describe an experiment to determine the amount of...Ch. 37 - Prob. 3S
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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