1 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781265486297
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 37, Problem 3S
Summary Introduction
To describe:
The factors in the water and air that is necessary to be monitored while growing the lettuce hydroponically in the greenhouse.
Introduction:
The hydroponics is the type of culture, in which the plants are grown in water instead of the soil. The nutrients and water continually flow through the roots of the plants. This allows the regulation of the amount of nutrients supplied to the plants.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 37 Solutions
1 SEM ACC W/RAVEN CARDED
Ch. 37.1 - Prob. 1LOCh. 37.1 - Prob. 2LOCh. 37.1 - Prob. 3LOCh. 37.2 - Prob. 1LOCh. 37.2 - Prob. 2LOCh. 37.2 - Describe the goal of food fortification research.Ch. 37.3 - Prob. 1LOCh. 37.3 - Prob. 2LOCh. 37.3 - Prob. 3LOCh. 37.4 - Prob. 1LO
Ch. 37.4 - Explain the main effect on herbivores of a higher...Ch. 37.4 - Prob. 3LOCh. 37.5 - Prob. 1LOCh. 37.5 - Prob. 2LOCh. 37.5 - Prob. 3LOCh. 37 - Prob. 1IQCh. 37 - Prob. 2IQCh. 37 - Which of the following is NOT found in topsoil? a....Ch. 37 - Prob. 2UCh. 37 - What proportion of the soil volume is occupied by...Ch. 37 - Which of the following is a micronutrient? a....Ch. 37 - Prob. 5UCh. 37 - Prob. 6UCh. 37 - a C4 plant, the Calvin cycle occurs in a. the...Ch. 37 - One potential problem with using poplars to remove...Ch. 37 - Prob. 1ACh. 37 - If you wanted to conduct an experiment to...Ch. 37 - Which of the following would decrease nitrogen...Ch. 37 - Which of the following might you do to increase...Ch. 37 - Prob. 5ACh. 37 - Prob. 1SCh. 37 - Describe an experiment to determine the amount of...Ch. 37 - Prob. 3S
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