Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Textbook Question
Chapter 36, Problem 2TYU
The cortical reaction of sea urchin eggs functions directly in
- A. the formation of a fertilization envelope.
- B. the production of a fast block to polyspermy.
- C. the generation of an electrical impulse by the egg.
- D. the fusion of egg and sperm nuclei.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 36 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 36.1 - Prob. 1CCCh. 36.1 - Prob. 2CCCh. 36.1 - MAKE CONNECTIONS What examples of plant...Ch. 36.2 - Prob. 1CCCh. 36.2 - Why might using a hot tub frequently make it...Ch. 36.2 - Prob. 3CCCh. 36.2 - WHAT IF? If each vas deferens in a male was...Ch. 36.3 - Prob. 1CCCh. 36.3 - How does an estrous cycle differ from a menstrual...Ch. 36.3 - Prob. 3CC
Ch. 36.4 - Prob. 1CCCh. 36.4 - Prob. 2CCCh. 36.4 - Prob. 3CCCh. 36 - Prob. 1TYUCh. 36 - The cortical reaction of sea urchin eggs functions...Ch. 36 - Prob. 3TYUCh. 36 - Prob. 4TYUCh. 36 - Prob. 5TYUCh. 36 - Which of the following statements is true? A. All...Ch. 36 - For which is the number the same in males and...Ch. 36 - Prob. 8TYUCh. 36 - Prob. 9TYUCh. 36 - Prob. 10TYUCh. 36 - FOCUS ON EVOLUTION Hermaphroditism is more often...Ch. 36 - Prob. 12TYUCh. 36 - Prob. 13TYU
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