Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Chapter 36.1, Problem 1CC
Summary Introduction
To determine:
The process of internal fertilization that facilitate life on land.
Introduction:
Internal fertilization involves the fusion of a sperm with an egg cell during sexual reproduction. Copulation takes place inside the body of the female parent. Male parent ejaculated their sperms in reproductive track of the female parent.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 36 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 36.1 - Prob. 1CCCh. 36.1 - Prob. 2CCCh. 36.1 - MAKE CONNECTIONS What examples of plant...Ch. 36.2 - Prob. 1CCCh. 36.2 - Why might using a hot tub frequently make it...Ch. 36.2 - Prob. 3CCCh. 36.2 - WHAT IF? If each vas deferens in a male was...Ch. 36.3 - Prob. 1CCCh. 36.3 - How does an estrous cycle differ from a menstrual...Ch. 36.3 - Prob. 3CC
Ch. 36.4 - Prob. 1CCCh. 36.4 - Prob. 2CCCh. 36.4 - Prob. 3CCCh. 36 - Prob. 1TYUCh. 36 - The cortical reaction of sea urchin eggs functions...Ch. 36 - Prob. 3TYUCh. 36 - Prob. 4TYUCh. 36 - Prob. 5TYUCh. 36 - Which of the following statements is true? A. All...Ch. 36 - For which is the number the same in males and...Ch. 36 - Prob. 8TYUCh. 36 - Prob. 9TYUCh. 36 - Prob. 10TYUCh. 36 - FOCUS ON EVOLUTION Hermaphroditism is more often...Ch. 36 - Prob. 12TYUCh. 36 - Prob. 13TYU
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