Prescott's Microbiology
Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 35.3, Problem 3RIA
Summary Introduction

In the vectors borne disease transmission, the microbes need a carrier to spread infections. The vectors act as a carrier in transmitting the infection from the infected individual to healthy individual or directly transmit disease to the healthy individual. For example, mosquito, ticks, bats, and dogs.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
When setting up a PCR reaction to act as a negative control for the surface protein A gene... Which primers will you add to the reaction mix? mecA primers, spa primers, mecA primers and spa primers, no primers What will you add in place of template? sterile water, MRSA DNA, Patient DNA, S. aureus DNA
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