Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 35, Problem 6CHI
Summary Introduction
Ebola virus disease (EVD), also termed as Ebola hemorrhagic fever (EHF), is a deadly hemorrhagic fever of humans and it is caused by Ebola virus. Common symptoms include abrupt fever, joint pain, sore throat, weakness, muscle aches, and headache. Progression of Ebola symptoms such as hiccups, vomiting, diarrhea, stomach pain, and rash. It is mainly transmitted through direct contact with body fluids like blood and secretions.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 35 Solutions
Prescott's Microbiology
Ch. 35.1 - MICRO INQUIRY What physical and chemical methods...Ch. 35.1 - MICRO INQUIRY During which stages does the host...Ch. 35.1 - Define infection, infectious disease,...Ch. 35.1 - What factors determine the outcome of most...Ch. 35.1 - Why would a psychrophile not be a human pathogen?Ch. 35.1 - What are some important characteristics of a...Ch. 35.1 - Prob. 5RIACh. 35.1 - How would you determine the ID50 of a suspected...Ch. 35.1 - What is an obligate intracellular pathogen? How...Ch. 35.1 - Prob. 8RIA
Ch. 35.2 - Describe several specific adhesins by which...Ch. 35.2 - What are virulence factors?Ch. 35.2 - What are pathogenicity islands and why are they...Ch. 35.2 - Prob. 4RIACh. 35.2 - What is a biofilm? How is it a virulence factor?Ch. 35.3 - Prob. 1RIACh. 35.3 - Define droplet nuclei, vehicle, fomite, and...Ch. 35.3 - Prob. 3RIACh. 35 - Prob. 1CHICh. 35 - Prob. 2CHICh. 35 - Prob. 3CHICh. 35 - Prob. 4CHICh. 35 - Prob. 5CHICh. 35 - Prob. 6CHI
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