1 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781265486297
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 35.1, Problem 2LO
Summary Introduction
To explain: That why meristem are vital after germination.
Introduction: The plants are mainly multicellular, predominantly photosynthetic eukaryotes of the kingdom Plantae. The meristem is a tissue in the plants that consists of undifferentiated cells, which is capable of cell division.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 35 Solutions
1 SEM ACC W/RAVEN CARDED
Ch. 35.1 - Prob. 1LOCh. 35.1 - Prob. 2LOCh. 35.1 - Prob. 3LOCh. 35.2 - Name the three cell types found in ground tissue...Ch. 35.2 - Prob. 2LOCh. 35.3 - Prob. 1LOCh. 35.3 - Prob. 2LOCh. 35.3 - Describe functions of modified roots.Ch. 35.4 - Prob. 1LOCh. 35.4 - Prob. 2LO
Ch. 35.4 - Prob. 3LOCh. 35.5 - Prob. 1LOCh. 35.5 - Prob. 2LOCh. 35 - Prob. 1DACh. 35 - Prob. 1IQCh. 35 - Which cells lack living protoplasts at maturity?...Ch. 35 - Prob. 2UCh. 35 - Prob. 3UCh. 35 - Prob. 4UCh. 35 - Prob. 5UCh. 35 - Prob. 6UCh. 35 - The function of guard cells is to a. allow carbon...Ch. 35 - Palisade and spongy parenchyma are typically found...Ch. 35 - Prob. 9UCh. 35 - Prob. 10UCh. 35 - Prob. 11UCh. 35 - Prob. 1ACh. 35 - Prob. 2ACh. 35 - If you were to relocate the pericycle of a plant...Ch. 35 - Prob. 4ACh. 35 - Prob. 5ACh. 35 - Prob. 6ACh. 35 - Prob. 7ACh. 35 - Prob. 8ACh. 35 - Which is the correct sequence of cell types...Ch. 35 - Prob. 10ACh. 35 - Prob. 1SCh. 35 - Prob. 2SCh. 35 - Prob. 3SCh. 35 - Prob. 4SCh. 35 - Increasing human population on the planet is...
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