CAMPBELL BIOLOGY-MASTERING BIO.ACCESS
12th Edition
ISBN: 9780136486787
Author: Urry
Publisher: SAVVAS L
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Chapter 35, Problem 6TYU
Summary Introduction
Introduction:
The pericycle is a unique primary tissue that is situated most outward of the root vascular cylinder in the stele region of plants. Pericycle consists of parenchymatous or sclerenchymatous cells. Its cells continue to cycle for a long period, and it has three functions.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 35 Solutions
CAMPBELL BIOLOGY-MASTERING BIO.ACCESS
Ch. 35.1 - Prob. 1CCCh. 35.1 - WHAT IF? If humans were photoautotrophs, making...Ch. 35.1 - Prob. 3CCCh. 35.2 - Prob. 1CCCh. 35.2 - Prob. 2CCCh. 35.2 - Prob. 3CCCh. 35.3 - Prob. 1CCCh. 35.3 - Prob. 2CCCh. 35.3 - Prob. 3CCCh. 35.4 - A sign is hammered into a tree 2 m from the tree's...
Ch. 35.4 - Prob. 2CCCh. 35.4 - Would you expect a tropical tree to have distinct...Ch. 35.4 - Prob. 4CCCh. 35.5 - How can two cells in a plant have vastly different...Ch. 35.5 - Prob. 2CCCh. 35.5 - Prob. 3CCCh. 35 - Prob. 35.1CRCh. 35 - Prob. 35.2CRCh. 35 - Prob. 35.3CRCh. 35 - Whht advantages did plants gain from the evolution...Ch. 35 - Prob. 35.5CRCh. 35 - Most of the growth of a plant body is the result...Ch. 35 - Prob. 2TYUCh. 35 - Prob. 3TYUCh. 35 - The phase change of an apical meristem from the...Ch. 35 - Prob. 5TYUCh. 35 - Prob. 6TYUCh. 35 - Prob. 7TYUCh. 35 - Supposc a flower had normal expression of genes A...Ch. 35 - Prob. 9TYUCh. 35 - Which of the following would not be seen in a...Ch. 35 - Prob. 11TYUCh. 35 - Prob. 12TYUCh. 35 - Prob. 13TYUCh. 35 - EVOLUTION CONNECTION Evolutionary biologists have...Ch. 35 - SCIENTIFIC INQUIRY Grasslands typically do not...Ch. 35 - SCIENCE, TECHNOLOGY, AND SOCIETY Hunger and...Ch. 35 - WRITE ABOUT A THEME: ORGANIZATION In a short essay...Ch. 35 - SYNTHESIZE YOUR KNOWLEDGE This stained light...
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