Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Chapter 35, Problem 10TYU
Summary Introduction
To determine:
The feasibility of treating septic shock with the drug that blocks the TLR signaling using mutated mice that has mutation that cause the inactivation of the TLR gene and purified LPS.
Introduction:
TLR are the toll like receptors that are involved in the septic shock of the body that is induced by the lipopolysaccharides (LPS) of the gram negative bacteria when it is introduced into the blood. The TLR are the reason for the septic shock.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 35 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 35.1 - Pus is both a sign of infection and an indicator...Ch. 35.1 - MAKE CONNECTIONS How do the molecules that...Ch. 35.1 - Prob. 3CCCh. 35.2 - Prob. 1CCCh. 35.2 - Explain how memory cells strengthen the immune...Ch. 35.2 - WHAT IF? If both copies of a light-chain gene and...Ch. 35.3 - Prob. 1CCCh. 35.3 - Prob. 2CCCh. 35.3 - Prob. 3CCCh. 35 - Prob. 1TYU
Ch. 35 - Prob. 2TYUCh. 35 - Prob. 3TYUCh. 35 - Prob. 4TYUCh. 35 - Prob. 5TYUCh. 35 - Prob. 6TYUCh. 35 - Prob. 7TYUCh. 35 - DRAW IT Consider a pencil-shaped protein with two...Ch. 35 - MAKE CONNECTIONS Contrast clonal selection with...Ch. 35 - Prob. 10TYUCh. 35 - FOCUS ON EVOLUTION Describe one invertebrate...Ch. 35 - Prob. 12TYUCh. 35 - Prob. 13TYU
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
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