CAMPBELL BIOLOGY-MASTERING BIO.ACCESS
12th Edition
ISBN: 9780136486787
Author: Urry
Publisher: SAVVAS L
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Chapter 34, Problem 34.7CR
Summary Introduction
To understand: The changes that occur in Hominin features based on fossil evidence.
Introduction: Hominin can be defined as a group consisting of modern humans, extinct humans, and their ancestors.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 34 Solutions
CAMPBELL BIOLOGY-MASTERING BIO.ACCESS
Ch. 34.1 - Identify four derived characters that all...Ch. 34.1 - Prob. 2CCCh. 34.1 - VISUAL SKILLS Based on the phylogenetic tree...Ch. 34.2 - How are differences in the anatomy of lampreys and...Ch. 34.2 - WHAT IF? In several different animal lineages,...Ch. 34.2 - WHAT IF? Suggest key roles that mineralized bone...Ch. 34.3 - What derived characters do sharks and tuna share?...Ch. 34.3 - Describe key adaptations of aquatic gnathostomes.Ch. 34.3 - draw it Redraw Figure 34.2 to show four lineages:...Ch. 34.3 - WHAT IF? Imagine that we could replay the history...
Ch. 34.4 - Prob. 1CCCh. 34.4 - Some amphibians never leave the water, whereas...Ch. 34.4 - WHAT IF? Scientists think that amphibian...Ch. 34.5 - Describe three key amniote adaptations for life on...Ch. 34.5 - Prob. 2CCCh. 34.5 - Identify four avian adaptations for flight.Ch. 34.5 - VISUAL SKILLS Based on the phylogeny shown in...Ch. 34.6 - Prob. 1CCCh. 34.6 - Prob. 2CCCh. 34.6 - MAKE CONNECTIONS Develop a hypothesis to explain...Ch. 34.7 - Identify some characters that distinguish hominins...Ch. 34.7 - Provide an example in which different features of...Ch. 34.7 - WHAT IF? Some genetic studies suggest that the...Ch. 34 - Describe likely features of the common ancestor of...Ch. 34 - Identify the shared features of early fossil...Ch. 34 - How would the appearance of organisms with jaws...Ch. 34 - Which features of amphibians restrict most species...Ch. 34 - Explain why birds are considered reptiles.Ch. 34 - Describe the origm and early evolution of mammals.Ch. 34 - Prob. 34.7CRCh. 34 - Vertebrates and tunicates share (A) jaws adapted...Ch. 34 - Living vertebrates can be divided into two major...Ch. 34 - Unlike eutherians, both monotremes and marsupials...Ch. 34 - Prob. 4TYUCh. 34 - As hominins diverged from other primates, which of...Ch. 34 - Which of the following could be considered the...Ch. 34 - Prob. 7TYUCh. 34 - SCIENTIFIC INQUIRY DRAW IT As a consequence of...Ch. 34 - Prob. 9TYUCh. 34 - SYNTHESIZE YOUR KNOWLEDGE This animal is a...
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