Nester's Microbiology: A Human Perspective
9th Edition
ISBN: 9781259709999
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 30, Problem 8SA
Summary Introduction
To review:
The sequence of events leading to botulism.
Introduction:
Botulism is a serious deadly illness due to the consumption of a neurotoxin called botulinum. This toxin is produced by the bacterium Clostridium botulinum. The toxin ingested by the person suffers from paralysis and have difficulty in speaking, in moving facial muscles, and swallowing.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 30 Solutions
Nester's Microbiology: A Human Perspective
Ch. 30 -
1. What is the purpose of rennin in...Ch. 30 - What causes bluish-green veins to form in some...Ch. 30 - What causes the holes to form in Swiss cheese?Ch. 30 - Why are starter cultures used when making semidry...Ch. 30 - What is the purpose of the mashing step in...Ch. 30 - Explain how Alcaligenes species cause “ropiness”...Ch. 30 - Explain the significance of Aspergillus flavus in...Ch. 30 - Prob. 8SACh. 30 -
9. Explain the typical sequence of events that...Ch. 30 - How does canning differ from pasteurization? page...
Ch. 30 - Prob. 1MCCh. 30 - Prob. 2MCCh. 30 - What is a generally minimum pH for growth and...Ch. 30 - Prob. 4MCCh. 30 - Prob. 5MCCh. 30 - Prob. 6MCCh. 30 -
7. Which of the following genera is used in...Ch. 30 -
8. Which group of organisms most commonly spoils...Ch. 30 - Prob. 9MCCh. 30 - Canned pickles require less stringent heat...Ch. 30 - Prob. 1ACh. 30 - Prob. 2ACh. 30 - Prob. 1CTCh. 30 -
2. In the production of sauerkraut, a natural...
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