Fundamentals of Biochemistry: Life at the Molecular Level
Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 3, Problem 37CQ
Summary Introduction

To determine: The two 12 residue primers that could be used to amplify the given DNA segment by PCR.

Concept introduction: The primers are small pieces of single stranded DNA segment which are complementary to the 3ʹ end of a polynucleotide and the hybridized primer is bound by the DNA polymerase. Then the polymerase is added nucleotides complementary to the template strand and the DNA molecules are lengthened in the 5ʹ to 3ʹ direction.

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Fundamentals of Biochemistry: Life at the Molecular Level

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