Fundamentals of Biochemistry: Life at the Molecular Level
Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 3, Problem 35CQ

(a)

Summary Introduction

To describe:  The possible products of a PCR in which one of the primers is advertently lost from the reaction mixture.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase, such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.

(b)

Summary Introduction

To describe:  The possible products of a PCR experiment in which one of the primers is complementary to several sites in the starting DNA sample.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.

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Fundamentals of Biochemistry: Life at the Molecular Level

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