Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 3, Problem 19E
Summary Introduction
To determine: The restriction enzymes would interfere with the production of β-galactosidase by the host cell.
Concept introduction: The plasmid pUC18 is a circular plasmid that contains multiple restriction sites. In pUC18, the enzyme β-galactosidase is encoded by the lacZ gene. The β-galactosidase cleaves the colorless compound X-gal to a blue-colored product.
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Solution
? Hint
Sub
bo Each pictured Lewis structure is invalid. Identify the error in each case.
O Macmillan Learning
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Answer Bank
wrong electron total
:0-
:F======F:
octet-rule violation
N
:0:
[s] mM
V (M/s)
Uninhibited
0.333
1.65 x 107
1.05 x 107
V (M/s) x 10'
Inhibitor A
V (M/s) x 107
Inhibitor B
0.794 x 107
0.40
1.86 x 107
1.21 x 107
0.893 x 107
0.50
2.13 x 107
1.43 x 107
1.02 x 107
0.666
2.49 x 107
1.74 x 107
1.19 x 107
1.0
2.99 x 107
2.22 x 107
1.43 x 107
2.0
3.72 x 107
3.08 x 107
1.79 x 107
For a Michaelis-Menten reaction, k₁-5 x 10'/M-s, k.-2 x 10%/s, and k₂-4 x 10²/s.
a)
Calculate the Ks and KM for this reaction.
b)
Does substrate binding achieve equilibrium or steady state?
Chapter 3 Solutions
Fundamentals of Biochemistry: Life at the Molecular Level
Ch. 3 - Prob. 1ECh. 3 - 2. Name the following nucleotide.
Ch. 3 - 3. In many organisms, DNA is modified by...Ch. 3 - 4. When cytosine is treated with bisulfite, the...Ch. 3 - 5. Kinases are enzymes that transfer a phosphoryl...Ch. 3 - 6. Kinases are enzymes that transfer a phosphoryl...Ch. 3 - 7. A diploid organism with a 45,000-kb haploid...Ch. 3 - 8. A segment of DNA containing 20 base pairs...Ch. 3 - 9. Explain why the strands of a DNA molecule can...Ch. 3 - 10. Explain why increasing the NaCl concentration...
Ch. 3 - 11. An enzyme from the human immunodeficiency...Ch. 3 - 12. How many different amino acids could...Ch. 3 - 13. The human genome contains thousands of...Ch. 3 - 14. By how many nucleotides, on average, do the...Ch. 3 - 15. The recognition sequence for the restriction...Ch. 3 - 16. Using the data in Table 3-2, identify...Ch. 3 - 17. The 13-Mb genome of the green alga...Ch. 3 - 18. Describe how to select recombinant clones if a...Ch. 3 - Prob. 19ECh. 3 - Prob. 20ECh. 3 - 21. Why is a genomic library larger than a cDNA...Ch. 3 - 22. Why do cDNA libraries derived from different...Ch. 3 - 23. Some RNA molecules me covalently modified by...Ch. 3 - 24. Would the modified nucleoside described in...Ch. 3 - 25. Draw the tautomeric form of adenine.
Ch. 3 - 26. Draw the tautomeric form of cytosine.
Ch. 3 - 27. The pK value for N1 of adenine is 3.64,...Ch. 3 - Prob. 28CQCh. 3 - Prob. 29CQCh. 3 - 30. Hypoxanthine can also base-pair with cytosine....Ch. 3 - 31. Describe the outcome of a chain-terminator...Ch. 3 - 32. Describe the outcome of a chain-terminator...Ch. 3 - 33. Calculate the number of clones required to...Ch. 3 - 34. You are attempting to clone a 250-kb segment...Ch. 3 - 35. Describe the possible outcome of a PCR...Ch. 3 - 36. Describe the possible outcome of a PCR...Ch. 3 - 37. Write the sequences of the two 12-residue...Ch. 3 - 38. A blood slain from a crime scene and blood...Ch. 3 - Prob. 1MTE
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