Chapter 3, Problem 14P

Summary Introduction

To calculate: The fraction of tmRNA bound for SmpB concentrations equal to

${10}^4{k}_d,{10}^3{k}_d,{10}^2{k}_d,{10}^1{k}_d,k_d,{10}^{-1}{k}_d,{10}^{-2}{k}_d,{10}^{-3}{k}_d,and{10}^{-4}{k}_d,$

Introduction: the protein SmpB binds to a special species of tRNA, tm RNA, to eliminate the incomplete proteins made from truncated mRNAs in bacteria. The relationship between fraction bound and concentration of SmpB can give useful information of relationship between K_d and concentration.