Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Textbook Question
Chapter 29, Problem 7TYU
Two groups of tomatoes were grown in the laboratory, one with humus added to the soil and the other a control without humus. The leaves of the plants grown without humus were yellowish (less green) compared with those of the plants grown in humus-enriched soil. The best explanation for this difference is that
- A. the healthy plants used carbohydrates in the decomposing leaves of the humus for energy to make chlorophyll.
- B. the humus made the soil more loosely packed, so water penetrated more easily to the roots.
- C. the humus contained minerals such as magnesium and iron, needed for the synthesis of chlorophyll.
- D. the heat released by the decomposing leaves of the humus caused more rapid growth and chlorophyll synthesis.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 29 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 29.1 - Why is long-distance transport important for...Ch. 29.1 - Prob. 2CCCh. 29.1 - WHAT IF? Some plants can detect increased levels...Ch. 29.2 - If a plant cell immersed in distilled water has a...Ch. 29.2 - How would a reduction in the number of aquaporin...Ch. 29.2 - WHAT IF? What would happen if you put plant...Ch. 29.3 - Prob. 1CCCh. 29.3 - WHAT IF? If an element increases the growth rate...Ch. 29.3 - Prob. 3CCCh. 29.4 - Prob. 1CC
Ch. 29.4 - Prob. 2CCCh. 29.4 - Prob. 3CCCh. 29.5 - A scientist adds a water-soluble inhibitor of...Ch. 29.5 - WHAT IF? Suppose an Arabidopsis mutant lacking...Ch. 29.5 - Prob. 3CCCh. 29.6 - Prob. 1CCCh. 29.6 - WHAT IF? If you buy cut flowers, why might the...Ch. 29.6 - Prob. 3CCCh. 29.7 - Prob. 1CCCh. 29.7 - Prob. 2CCCh. 29.7 - Prob. 3CCCh. 29 - Which structure or compartment is part of the...Ch. 29 - Prob. 2TYUCh. 29 - Prob. 3TYUCh. 29 - What would enhance water uptake by a plant cell?...Ch. 29 - Prob. 5TYUCh. 29 - Compared with a cell with few aquaporin proteins...Ch. 29 - Two groups of tomatoes were grown in the...Ch. 29 - Prob. 8TYUCh. 29 - Prob. 9TYUCh. 29 - SCIENTIFIC INQUIRY A Minnesota gardener notes that...Ch. 29 - Prob. 11TYUCh. 29 - FOCUS ON INTERACTIONS The soil in which plants...Ch. 29 - Prob. 13TYU
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