Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 28.5, Problem 2MQ
- Describe the method, time frame, and rationale for the tuberculin skin test. What component of the immune response does this test detect?
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Describe the method, time frame, and rationale for the tuberculinskin test. What component of the immune response does thistest detect?
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Question 28 options:
a)
HLA antigens move through a limited pH gradient and are immobilized, complexed with antibody, and stained.
b)
T lymphocytes are harvested and introduced to a panel of test sera specific for antigens of the HLA system.
c)
DNA is isolated from peripheral white blood cells, fragmented, and hybridized.
d)
B lymphocytes are harvested and introduced to a panel of test sera specific for antigens of the HLA system.
Chapter 28 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 28.1 - The use of personal protective equipment (PPE) is...Ch. 28.1 - Identify and discuss the standard safety...Ch. 28.1 - Prob. 1CRCh. 28.2 - Prob. 1MQCh. 28.2 - How can the spread of HAIs be controlled?Ch. 28.2 - Prob. 1CRCh. 28.3 - What are the key points necessary for proper...Ch. 28.3 - Identify culture methods and conditions used for...Ch. 28.3 - QWhy is it important to process clinical specimens...Ch. 28.4 - Describe the disc diffusion test and the Etest for...
Ch. 28.4 - What is the value of antimicrobial drug...Ch. 28.4 - QDescribe the disc diffusion test for antibiotic...Ch. 28.5 - Explain the reasons for changes in antibody titer...Ch. 28.5 - Describe the method, time frame, and rationale for...Ch. 28.5 - What advantages do monoclonal antibodies have...Ch. 28.5 - QWhy does antibody titer rise after infection? Is...Ch. 28.6 - How is the bivalence of antibodies significant for...Ch. 28.6 - What are the advantages and disadvantages of...Ch. 28.6 - Why are agglutination tests so widely used in...Ch. 28.7 - Prob. 1MQCh. 28.7 - Compare the advantages and disadvantages of EIA,...Ch. 28.7 - Prob. 1CRCh. 28.8 - What advantage(s) does nucleic acid amplification...Ch. 28.8 - How do quantitative PCR (qPCR) and qualitative PCR...Ch. 28.8 - Distinguish between quantitative and qualitative...Ch. 28.9 - Compare and contrast live attenuated vaccines,...Ch. 28.9 - Identify the advantages of alternative...Ch. 28.9 - QList the immunizations recommended for children...Ch. 28.10 - Prob. 1MQCh. 28.10 - How does the activity of each antibiotic class...Ch. 28.10 - What are the sources of aminoglycosides,...Ch. 28.10 - Antibiotics are chemically diverse antimicrobial...Ch. 28.11 - What steps in the viral maturation process are...Ch. 28.11 - Why are there fewer clinically effective...Ch. 28.11 - Why is host toxicity a common problem with...Ch. 28.12 - Identify the basic mechanisms of antibiotic...Ch. 28.12 - What does vancomycin have in common with...Ch. 28.12 - Prob. 3MQCh. 28.12 - What practices contribute to the spread of...Ch. 28 - Define the procedures you would use to isolate and...Ch. 28 - Prob. 2AQCh. 28 - Describe three important reasons why semisynthetic...Ch. 28 - Imagine yourself as a clinical microbiologist with...
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- Antibodies can be divided into monoclonal and polyclonal. State the main difference between them. Which type of antibodies is more preferable in routine bioanalysis? Explain your answer.arrow_forwardIs rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.arrow_forwardCompare and contrast Radial Immunodiffusion with it’s close relative, the Ouchterlony plate technique. How are they similar and how are they different?arrow_forward
- How is a direct agglutination test different from a passive agglutination test?arrow_forwardAn ELISA was performed on the sera of three patients to detect antibody against varicella (the chickenpox). Give the titer results shown in the answers, which patient's serum had the most anti-varicella antibody? There is not enough information to answer the question. Patient 2, dilution 1:30 O Patient 1, dilution 1:2,000 O Patient 2, dilution 1:400arrow_forwardIn this diagram, why is anti-human IgG used in the indirect test but not in the direct test?arrow_forward
- What are the major differences between an IgE-mediated skin reaction, such as hives, and a delayed-type hypersensitivity reaction, such as a positive tuberculin skin test?arrow_forwardTo diagnosis, we used a procedure known as a "sandwich ELISA" (Enzyme-Linked Immunosorbent Assay). 1.In the "ELISA sandwich," what is the "meat"? 2.Both patients A and B had positive results. Why are these two patients' outcomes so different? 3.What is one potential cause of an ELISA false negative result? What is one potential cause of an ELISA false positive result? What is another example of how an ELISA assay should be used?arrow_forwardWhat is immunoassay? Differentiate direct and indirect immunoasay tests.arrow_forward
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