MICROBIOLOGY-MASTERING MICRO.-ACCESS
MICROBIOLOGY-MASTERING MICRO.-ACCESS
11th Edition
ISBN: 9780321802705
Author: Tortora
Publisher: PEARSON
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Chapter 28, Problem 1CAE

Suppose you are culturing a microorganism that produces enough lactic acid to kill itself in a few days.

  1. a. How can the use of a bioreactor help you maintain the culture for weeks or months? The graph below shows conditions in the bioreactor:

Chapter 28, Problem 1CAE, Suppose you are culturing a microorganism that produces enough lactic acid to kill itself in a few

  1. b. If your desired product is a secondary metabolite, when can you begin collecting it?
  2. c. If your desired product is the cells themselves and you want to maintain a continuous culture, when can you begin harvesting?
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Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
If a biotechnologist wishes to obtain a primary metabolite, they will harvest the organism/media during log phase. If a biotechnologist wishes to obtain a secondary metabolite, they will harvest the organism/media during stationary phase. Can you explain this distinction regarding the metabolism of the cell at these two different batch culture growth phases?
3b)A plant suspension culture was used to study the effect of three types of media on the growth of the culture using batch culture approach. Figure 3.1 shows the growth of the plant suspension cultures using Murashige and Skoog (MS), Gamborg B5 and Vacin and Went media. Comment and conclude on the results obtained. Suggest which is the most suitable medium to maintain the suspension cultures with justification.

Chapter 28 Solutions

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cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY