Mannitol salt agar (MSA) only allows the growth of halophiles. Among the halophiles, mannitol fermenters release acid that turns the pH indicator yellow; mannitol non-fermenters leave the medium red. Onto MSA you inoculate a halophilic mannitol non-fermenter and a non-halophilic mannitol non-fermenter. What are your expected results in this experiment? Explain the role of the media in this case.

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.