Prescott's Microbiology
Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 27.2, Problem 1.3RIA

How is a prophage induced to become active again?

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. Consider a base substitution mutation that occurred in a DNA sequence that resulted in a change in the encoded protein from the amino acid glutamic acid to aspartic acid. Normally the glutamic acid amino acid is located on the outside of the soluble protein but not near an active site. O-H¨ A. What type of mutation occurred? O-H B. What 2 types of chemical bonds are found in the R-groups of each amino acid? The R groups are shaded. CH2 CH2 CH2 H2N-C-COOH H2N-C-COOH 1 H Glutamic acid H Aspartic acid C. What 2 types of bonds could each R-group of each of these amino acids form with other molecules? D. Consider the chemical properties of the two amino acids and the location of the amino acid in the protein. Explain what effect this mutation will have on this protein's function and why.
engineered constructs that consist of hollow fibers are acting as synthetic capillaries, around which cells have been loaded. The cellular space around a single fiber can be modeled as if it were a Krogh tissue cylinder. Each fiber has an outside “capillary” radius of 100 µm and the “tissue” radius can be taken as 200 µm. The following values apply to the device:R0 = 20 µM/secaO2 = 1.35 µM/mmHgDO2,T = 1.67 x 10-5 cm2/secPO2,m = 4 x 10-3 cm/secInstead of blood inside the fibers, the oxygen transport and tissue consumption are being investigated by usingan aqueous solution saturated with pure oxygen. As a result, there is no mass transfer resistance in the synthetic“capillary”, only that due to the membrane itself. Rather than accounting for pO2 variations along the length ofthe fiber, use an average value in the “capillary” of 130 mmHg.Is the tissue fully oxygenated?

Chapter 27 Solutions

Prescott's Microbiology

Ch. 27.2 - How do the temperate phages Mu and P1 differ from...Ch. 27.2 - The CRISPR/Cas system has been a boon to...Ch. 27.2 - Why do cold sores recur throughout the lifetime of...Ch. 27.2 - In what part of the host cell does a herpesvirus...Ch. 27.2 - Many small DNA viruses rely on host enzymes for...Ch. 27.3 - Why is the X174 genome considered plus stranded?Ch. 27.3 - Prob. 2MICh. 27.3 - Why is it necessary for some ssDNA viruses to...Ch. 27.3 - From the point of view of the virus, compare the...Ch. 27.3 - How do parvoviruses trick the host DNA polymerase...Ch. 27.4 - The rotavirus genome encodes 12 proteins. Suggest...Ch. 27.4 - Describe the life cycle of 6 phage. What makes...Ch. 27.4 - Prob. 3RIACh. 27.4 - In what ways are the life cycles of 6 and...Ch. 27.5 - Where in the host does the plus-strand RNA genome...Ch. 27.5 - How do some plus-strand viruses use polyproteins...Ch. 27.5 - What is an IRES? Why is it important?Ch. 27.5 - Prob. 3RIACh. 27.6 - How does that use of a segmented genome by...Ch. 27.6 - Prob. 2RIACh. 27.7 - Prob. 1MICh. 27.7 - Prob. 1RIACh. 27.7 - Prob. 2RIACh. 27.7 - What role does alternative splicing play in the...Ch. 27.8 - Prob. 1RIACh. 27.8 - Trace the HBV multiplication cycle, paying...Ch. 27 - No temperate RNA phages have yet been discovered....Ch. 27 - The choice between lysogeny and lysis is...Ch. 27 - Prob. 3CHICh. 27 - You are studying RNA viruses and have discovered a...Ch. 27 - Prob. 5CHICh. 27 - Upon infection of host epithelial cells,...Ch. 27 - Associated with the envelope of herpesviruses are...
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