Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Textbook Question
Chapter 27, Problem 9RQ
An animal whose development is marked by radial cleavage and enterocoely is.
- a deuterostome
- an annelid or mollusk
- either an acoelomate or eucoelomate
- none of the above
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 27 Solutions
Biology 2e
Ch. 27 - Figure 27.5 If a Hox 13 gene in a mouse was...Ch. 27 - Figure 27.6 Which of the following statements is...Ch. 27 - Figure 27.9 Which of the following statements...Ch. 27 - Which of the following is not a feature common to...Ch. 27 - During embryonic development, unique cell layers...Ch. 27 - Which of the following phenotypes would most...Ch. 27 - Which of the following organisms is most likely to...Ch. 27 - Which of the following is not possible? radially...Ch. 27 - An animal whose development is marked by radial...Ch. 27 - Consulting the modern phylogenetic tree of...
Ch. 27 - Which of the following is thought to be the most...Ch. 27 - As with the emergence of the Acoelomorpha phylum,...Ch. 27 - Which of the following periods is the earliest...Ch. 27 - What type of data is primarily used to determine...Ch. 27 - The time between 542-488 million years ago marks...Ch. 27 - Until recent discoveries suggested otherwise,...Ch. 27 - Plant life first appeared on land during which of...Ch. 27 - Approximately how many mass extinction events...Ch. 27 - Why might the evolution of specialized tissues be...Ch. 27 - Describe and give examples of how humans display...Ch. 27 - How have Hox genes contributed to the diversity of...Ch. 27 - Using the following terms, explain what...Ch. 27 - Explain some of the advantages brought about...Ch. 27 - Describe at least two major changes to the animal...Ch. 27 - How is it that morphological data alone might lead...Ch. 27 - Briefly describe at least two theories that...Ch. 27 - How is it that most if not all, of the extant...
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