Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Chapter 27, Problem 19CTQ
Why might the evolution of specialized tissues be important for animal function and complexity?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 27 Solutions
Biology 2e
Ch. 27 - Figure 27.5 If a Hox 13 gene in a mouse was...Ch. 27 - Figure 27.6 Which of the following statements is...Ch. 27 - Figure 27.9 Which of the following statements...Ch. 27 - Which of the following is not a feature common to...Ch. 27 - During embryonic development, unique cell layers...Ch. 27 - Which of the following phenotypes would most...Ch. 27 - Which of the following organisms is most likely to...Ch. 27 - Which of the following is not possible? radially...Ch. 27 - An animal whose development is marked by radial...Ch. 27 - Consulting the modern phylogenetic tree of...
Ch. 27 - Which of the following is thought to be the most...Ch. 27 - As with the emergence of the Acoelomorpha phylum,...Ch. 27 - Which of the following periods is the earliest...Ch. 27 - What type of data is primarily used to determine...Ch. 27 - The time between 542-488 million years ago marks...Ch. 27 - Until recent discoveries suggested otherwise,...Ch. 27 - Plant life first appeared on land during which of...Ch. 27 - Approximately how many mass extinction events...Ch. 27 - Why might the evolution of specialized tissues be...Ch. 27 - Describe and give examples of how humans display...Ch. 27 - How have Hox genes contributed to the diversity of...Ch. 27 - Using the following terms, explain what...Ch. 27 - Explain some of the advantages brought about...Ch. 27 - Describe at least two major changes to the animal...Ch. 27 - How is it that morphological data alone might lead...Ch. 27 - Briefly describe at least two theories that...Ch. 27 - How is it that most if not all, of the extant...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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- Which of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have decided to send your sample off for sequencing. You need to determine which type of sequencing to use for the preliminary identification of your species. You decide that, for now, you only need to be able to identify the family and genus levels. Which type of sequencing do you think is the most appropriate? O Genomic Fingerprinting O Whole Genome Sequencing O 16S rDNA Sequencing O DNA-DNA hybridization Nextarrow_forward
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