The use of AMPK activation in fatty acid oxidation should be determined. Concept introduction: Fatty acid is an aliphatic carboxylic acid chain. It can be either saturated or unsaturated. Natural fatty acids have even number of carbon atoms (28-40), and the chain is unbranched in nature. They are very important in the diet, as they act as fuel for the biochemical reactions inside the body.

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Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 27, Problem 41P

Interpretation Introduction

Interpretation:

The use of AMPK activation in fatty acid oxidation should be determined.

Concept introduction:

Fatty acid is an aliphatic carboxylic acid chain. It can be either saturated or unsaturated. Natural fatty acids have even number of carbon atoms (28-40), and the chain is unbranched in nature. They are very important in the diet, as they act as fuel for the biochemical reactions inside the body.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

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Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 27, Problem 41P

Interpretation Introduction

Interpretation:

The use of AMPK activation in fatty acid oxidation should be determined.

Concept introduction:

Fatty acid is an aliphatic carboxylic acid chain. It can be either saturated or unsaturated. Natural fatty acids have even number of carbon atoms (28-40), and the chain is unbranched in nature. They are very important in the diet, as they act as fuel for the biochemical reactions inside the body.

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Answer 3

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Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 27, Problem 41P

Interpretation Introduction

Interpretation:

The use of AMPK activation in fatty acid oxidation should be determined.

Concept introduction:

Fatty acid is an aliphatic carboxylic acid chain. It can be either saturated or unsaturated. Natural fatty acids have even number of carbon atoms (28-40), and the chain is unbranched in nature. They are very important in the diet, as they act as fuel for the biochemical reactions inside the body.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 27, Problem 41P

Interpretation Introduction