Interpretation:
Percentages of each component should be calculated.
Concept introduction:
Area normalization method is used in quantitative analysis of compounds in chromatography. In this method of quantification, complete elution of all analytes in the sample is required. Following formulas are required for the calculation.
Answer to Problem 27.26QAP
Explanation of Solution
Given information:
Compound | Relative peak area | Relative detector response |
A | 32.5 | 0.70 |
B | 20.7 | 0.72 |
C | 60.1 | 0.75 |
D | 30.2 | 0.73 |
E | 18.3 | 0.78 |
Corrected areas of compound can be calculated as follows.
Now, the percentage of compound can be calculated as follows:
Or,
Also,
Thus,
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