Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Question
Chapter 27, Problem 27.19QAP
Interpretation Introduction
Interpretation:
The definition for the retention index and its method of determination is to be stated.
Concept introduction:
The retention index is used to determine the solutes from the chromatograms. It is derived from the mixture of solutes chromatogram. The solute must have two
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
List five factors that affect the retention time.
A student performed a chromatography
experiment to determine the Rf value of an
unknown amino acid. The distances traveled by
the amino acid and the solvent line were 3.0 cm
and 8.5 cm, respectively. What is the Rf value for
this amino acid?
0.3 cm
O 0.35
1.8
5.5
0.85 cm
0.65
O 2.8
Describe how to obtain a Retention Factor. What does the retention factor tell you?
Chapter 27 Solutions
Principles of Instrumental Analysis
Ch. 27 - Prob. 27.1QAPCh. 27 - Prob. 27.2QAPCh. 27 - Prob. 27.3QAPCh. 27 - Prob. 27.4QAPCh. 27 - Prob. 27.5QAPCh. 27 - Prob. 27.6QAPCh. 27 - Prob. 27.7QAPCh. 27 - Prob. 27.8QAPCh. 27 - Prob. 27.9QAPCh. 27 - What are hyphenated GC methods? Briefly describe...
Ch. 27 - Prob. 27.11QAPCh. 27 - Prob. 27.12QAPCh. 27 - Prob. 27.13QAPCh. 27 - Prob. 27.14QAPCh. 27 - Prob. 27.15QAPCh. 27 - Prob. 27.16QAPCh. 27 - Prob. 27.17QAPCh. 27 - Prob. 27.18QAPCh. 27 - Prob. 27.19QAPCh. 27 - The same polar compound is gas chromatographed on...Ch. 27 - Prob. 27.21QAPCh. 27 - Prob. 27.22QAPCh. 27 - Prob. 27.23QAPCh. 27 - Prob. 27.24QAPCh. 27 - Prob. 27.25QAPCh. 27 - Prob. 27.26QAPCh. 27 - Prob. 27.27QAPCh. 27 - Prob. 27.28QAPCh. 27 - Why is GSC not used nearly as extensively as GLC?Ch. 27 - Prob. 27.30QAP
Knowledge Booster
Similar questions
- Table 2: Absorbance Values of Standards and Unknowns. Sample Blank Standard Solution 1 Standard Solution 2 Standard Solution 3 Standard Solution 4 Standard Solution 5 Water Sample 1 Water Sample 2 Phosphate Concentration (in ppm) Y17 0 ppm 0.02 ppm 0.04 ppm 0.08 ppm 0.16 ppm 0.32 ppm Freeman Lake 0.001 0.325 0.292 0.413 0.315 0.039 0.054 0.049 Absorbance at 2= 690 nm Calculations: 1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes on the graph and provide the curve with a title. Use a linear trendline to generate a best fit line to your data. Label the graph with the equation and the R" value. Insert your labeled graph in the space below. Xarrow_forwardIn normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.arrow_forward▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? A) methanol B) Ethanol chu C) IPAarrow_forward
- What types of samples can be analyzed by IR?How are substances that are opaque (nottransparent) handled using this technique?arrow_forwardThe concentration of methylene blue in the sample is estimated through (select the correct) options: refractive index measurement (refractometry). turbidity measurement (turbidimetry). absorbance measurement (spectrophotometry). titration with 2% HCl solution.arrow_forwardWhat is the retention factor (Rf) of the most polar and least polar spot present in the standard ink? (Use 2 significant figures)arrow_forward
- use the following chromatogram and table as your data. What is the %composition of the Vitamin A peak in the sample? 78.43% 77.73% 7.867% 72.74%arrow_forwardIn a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.arrow_forwardSolution Concentration of FeSCN2+ (M) Absorbance1 0.0000491 0.3112 0.0000818 0.5763 0.000115 0.7554 0.000152 0.9075 0.000196 1.185 Solution Concentration of FeSCN2+ (M) Absorbance 1 0.0000491 0.311 2 0.0000818 0.576 3 0.000115 0.755 4 0.000152 0.907 5 0.000196 1.185 A series of aqueous solutions of FeSCN2+ of different concentrations were prepared, and the absorbance of each was measured at a wavelength of 455 nm. Use Excel or Google Sheets to prepare a useful graph of the data (given below) that could be used to determine the concentration of an unknown solution from its absorbance. Ensure your graph is properly formatted and labeled. Click the…arrow_forward
- What is the importance of paper chromotography? What is the usage of paper chromotography in the food industry? Who invented paper chromotography?arrow_forwardQ. Determination of hardness by Clark's method.arrow_forwardPre-Lab Questions Experiment 12 Food Chromatography: The Distance Our Food Travels Name: Section: Instructor: Date: Imagine you have a solution that is comprised solely of component A. You have another solution that is comprised solely of component B, and yet a third solution that is comprised solely of component C. Component A's retention factor is about 0.84, Component B's retention factor is about 0.21, and Component C's retention factor is 0.55 in the same solvent system. You then have a fourth solution that is composed of components A and C. 145 You spot each solution on a piece of chromatography paper and allow the solutions to separate. Sketch what you think your paper would look like below.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning
Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning