Chapter 26.7, Problem 4TY

Interpretation Introduction

Interpretation:

The electro kinetic injection time should be explained, when decreasing of applied voltage by a factor of 2.

Concept introduction:

Electro kinetic injection:

In the capillary electrophoresis, the electro kinetic injection time is given by below equation,

$\begin{array}{l}\text{Time}\text{(t)}\text{=}\frac{\text{plug}\text{length}}{\text{speed}}\\ \text{=}\frac{\text{plug}\text{length}}{{\text{μ}}_{\text{app}}\left(\text{E}\frac{{\text{K}}_{\text{b}}}{{\text{K}}_{\text{s}}}\right)}\end{array}$

Where,

${\text{μ}}_{\text{app}}$ is the conductivity of the electrolyte

$\text{E}$ is the applied electric field

${\text{K}}_{\text{b}}$ is the conductivities of buffer

${\text{K}}_{\text{s}}$ is the conductivities of sample