The suitable chromatography technique should be given for purification of antibody. Affinity Chromatography: The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

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Answer 1

Question

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

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(a 4 shows scanning electron microscope (SEM) images of extruded actions of packing bed for two capillary columns of different diameters, al 750 (bottom image) and b) 30-μm-i.d. Both columns are packed with the same stationary phase, spherical particles with 1-um diameter. A) When the columns were prepared, the figure shows that the column with the larger diameter has more packing irregularities. Explain this observation. B) Predict what affect this should have on band broadening and discuss your prediction using the van Deemter terms. C) Does this figure support your explanations in application question 33? Explain why or why not and make any changes in your answers in light of this figure. Figure 4 SEM images of sections of packed columns for a) 750 and b) 30-um-i.d. capillary columns.³

fcrip = ↓ bandwidth Il temp 32. What impact (increase, decrease, or no change) does each of the following conditions have on the individual components of the van Deemter equation and consequently, band broadening? Increase temperature Longer column Using a gas mobile phase instead of liquid Smaller particle stationary phase Multiple Paths Diffusion Mass Transfer

34. Figure 3 shows Van Deemter plots for a solute molecule using different column inner diameters (i.d.). A) Predict whether decreasing the column inner diameters increase or decrease bandwidth. B) Predict which van Deemter equation coefficient (A, B, or C) has the greatest effect on increasing or decreasing bandwidth as a function of i.d. and justify your answer. Figure 3 Van Deemter plots for hydroquinone using different column inner diameters (i.d. in μm). The data was obtained from liquid chromatography experiments using fused-silica capillary columns packed with 1.0-μm particles. 35 20 H(um) 큰 20 15 90 0+ 1500 100 75 550 01 02 594 05 μ(cm/sec) 30 15 10

Answer 2

Question

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

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Answer 3

Question

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

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Answer 4

Question

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

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Answer 5

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Answer 6

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

Answer 7

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

Answer 8

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Answer 9

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Answer 10

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Answer 11

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Answer 12

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Answer 13

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 26.1 - Prob. 1TY Ch. 26.7 - Prob. 3TY Ch. 26.7 - Prob. 4TY Ch. 26 - Prob. 26.AE Ch. 26 - Prob. 26.BE Ch. 26 - Prob. 26.CE Ch. 26 - Prob. 26.DE Ch. 26 - Prob. 26.1P Ch. 26 - Prob. 26.2P Ch. 26 - Prob. 26.3P

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Chapter 26 Solutions