The suitable chromatography technique should be given for purification of antibody. Affinity Chromatography: The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

Question

Want to see the full answer?

Answer 1

Question

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Questions 4 and 5

For a titration of 40.00 mL of 0.0500 M oxalic acid H2C2O4 with 0.1000 M KOH, calculate the pH at each of the following volume of KOH used in the titration: 1) before the titration begin;2) 15 mL; 3) 20 mL; 4) 25 mL; 5) 40 mL; 6) 50 mL. Ka1 = 5.90×10^-2, Ka2 = 6.50×10^-5 for oxalic acid.

Predict the major organic product(s), if any, of the following reactions. Assume all reagents are in excess unless otherwise indicated.

Answer 2

Question

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Answer 6

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

Answer 7

Chapter 26, Problem 26.18P

(a)

Interpretation Introduction

Interpretation:

The suitable chromatography technique should be given for purification of antibody.

Affinity Chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity Chromatography.

Answer 8

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Answer 9

(b)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for desalting of protein should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Answer 10

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Answer 11

(c)

Interpretation Introduction

Interpretation:

The suitable chromatography technique for molar mass determination of polystyrene should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Answer 12

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Answer 13

(d)

Interpretation Introduction

Interpretation:

The suitable technique for separation of Cytochrome and Ribonuclease from its mixture should be given.

Molecular exclusion chromatography:

The molecules are separated by its size in chromatographic separation is known as molecular exclusion chromatography.

In this chromatography, the large molecules are eluted first then small molecules are separated.

The elution volume is directly proportional to the molar mass of the molecule so it is called as gel filtration or permeation chromatography.

Hydrophobic interaction chromatography:

The substance, which is repels the water is known as hydrophobic substance. In Hydrophobic interaction chromatography, the stationary phase is hydrophobic substance.

In the chromatography, the molecule is interact with stationary phase of the column and the solubility of molecule, which is going to separate is decreasing salt concentration of reagent ti increasing solubility molecule.

Affinity chromatography:

The sample mixture is passed through a column, only a single molecule is bounded to the stationary phase and other molecules are eluted out. After elution, the affinity of bonded molecules is realised, when pH of the column is varied. In the above chromatography affinity act as an important role, therefore it is called as affinity chromatography.

Answer 14

Expert Solution & Answer

Answer 15

Expert Solution & Answer

Answer 16

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 17

Ch. 26.1 - Prob. 1TY Ch. 26.7 - Prob. 3TY Ch. 26.7 - Prob. 4TY Ch. 26 - Prob. 26.AE Ch. 26 - Prob. 26.BE Ch. 26 - Prob. 26.CE Ch. 26 - Prob. 26.DE Ch. 26 - Prob. 26.1P Ch. 26 - Prob. 26.2P Ch. 26 - Prob. 26.3P

Want to see the full answer?

Chapter 26 Solutions