The separation of anion and cation by hydrophilic interaction chromatography should be explained. Concept introduction: Hydrophilic interaction chromatography: The samples, which are interaction with water and it dissolves in water and they are attract the water molecules in their surface in known as hydrophilic substances, this substances are mostly soluble in water and polar substances. In chromatographic separation of hydrophilic by substances chromatography, the sample is interact with the stationary phase so therefore, the mobile phase is taken into nonpolar to increasingly polar organic solvents. The hydrophilic interaction chromatography is working in the principle of mass action therefore, increasing of gradient ionic strength results the efficiency of separation.

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Chapter 26, Problem 26.11P

(a)

Interpretation Introduction

Interpretation:

The separation of anion and cation by hydrophilic interaction chromatography should be explained.

Concept introduction:

Hydrophilic interaction chromatography:

The samples, which are interaction with water and it dissolves in water and they are attract the water molecules in their surface in known as hydrophilic substances, this substances are mostly soluble in water and polar substances.

In chromatographic separation of hydrophilic by substances chromatography, the sample is interact with the stationary phase so therefore, the mobile phase is taken into nonpolar to increasingly polar organic solvents.

The hydrophilic interaction chromatography is working in the principle of mass action therefore, increasing of gradient ionic strength results the efficiency of separation.

(b)

Interpretation Introduction

Interpretation:

The work of given charged aerosol detector should be explained.

Concept introduction:

Charged aerosol detector:

The detector, which is detect (measure) the amount of charged substances in a sample by detection of passed aerosol particles is known as charged aerosol detector.

It is used as a universal detector for HPLC.

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