Chapter 26, Problem 26.37P

Interpretation Introduction

Interpretation:

The number of theoretical plates for the given asymmetric peak should be determined.

Concept introduction:

The number of theoretical plates for the electrophoretic peak:

In the capillary electrophoresis, the number of electrophoretic peak that obtained in capillary electrophoresis is given by below equation,

$\text{N}\text{=}\frac{16t_r{}^2}{w^2}$

Where,

$\text{N}$ is number of electrophoretic peak

$\text{w}$ is peak width

${\text{t}}_{\text{r}}$ is retention time.

The Foley-Dorsey equation:

The number of theoretical plates for the asymmetric peak is measured by using Foley-Dorsey equation it is,

$\text{N}\text{=}\frac{\text{41}{\text{.7(t}}_{\text{r}}{\text{/w}}_{\text{o}\text{.1}}{\text{)}}^{\text{2}}}{\text{(A/B+1}\text{.25)}}$

Where,

$\text{A/B}$ is asymmetry factor