Solution Manual for Quantitative Chemical Analysis
Solution Manual for Quantitative Chemical Analysis
9th Edition
ISBN: 9781464175633
Author: Daniel Harris
Publisher: Palgrave Macmillan Higher Ed
Question
Book Icon
Chapter 26, Problem 26.44P
Interpretation Introduction

Interpretation:

The plot should be drawn by using given data and molar masses of ferritin molecules should be calculated.

Concept introduction:

Molecular mass determination by Sodium dodecyl sulfate (SDS)-gel electrophoresis:

The movement of solute particle respect with applied electrical current is known as electrophoresis, and it is called as a capillary electrophoresis, when it is conducted in capillary tube.

The migration of molecule is depends on its molar mass, the larger molecules are slowly moves towards the electrodes than the smaller molar mass containing compounds.

From the above concept the molecular mass of proteins are determined by sodium dodecyl sulfate (SDS)-gel electrophoresis.  The denatured proteins are treated with dodecyl sulfate to make large negative charge respect with length of the protein chain.

The mass of the protein is proportional to 1/ (migration time).

The migration time of protein in the gel electrophoresis is used to calculate the molar mass of protein.

Blurred answer
Knowledge Booster
Background pattern image
Recommended textbooks for you
Text book image
Chemistry
Chemistry
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Cengage Learning
Text book image
Chemistry
Chemistry
ISBN:9781259911156
Author:Raymond Chang Dr., Jason Overby Professor
Publisher:McGraw-Hill Education
Text book image
Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning
Text book image
Organic Chemistry
Chemistry
ISBN:9780078021558
Author:Janice Gorzynski Smith Dr.
Publisher:McGraw-Hill Education
Text book image
Chemistry: Principles and Reactions
Chemistry
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:Cengage Learning
Text book image
Elementary Principles of Chemical Processes, Bind...
Chemistry
ISBN:9781118431221
Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:WILEY