The three properties that are common to the reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase. Introduction: RNA polymerase enzyme catalyzes the transcription process in which RNA is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

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Chapter 26, Problem 6P

Summary Introduction

To determine: The three properties that are common to the reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase.

Introduction:

RNA polymerase enzyme catalyzes the transcription process in which RNA is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Summary Introduction

To determine: The similarities and differences of polynucleotide phosphorylase with the above mentioned four enzymes (DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase).

Introduction:

Polynucleotide phosphorylase is a bifunctional enzyme that catalyzes the long chains of polyribonucleotide (RNA). RNA polymerase enzyme catalyzes the transcription process in which RNA polymerase is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 26, Problem 6P

Summary Introduction

To determine: The three properties that are common to the reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase.

Introduction:

RNA polymerase enzyme catalyzes the transcription process in which RNA is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Summary Introduction

To determine: The similarities and differences of polynucleotide phosphorylase with the above mentioned four enzymes (DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase).

Introduction:

Polynucleotide phosphorylase is a bifunctional enzyme that catalyzes the long chains of polyribonucleotide (RNA). RNA polymerase enzyme catalyzes the transcription process in which RNA polymerase is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

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Answer 3

Question

Chapter 26, Problem 6P

Summary Introduction

To determine: The three properties that are common to the reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase.

Introduction:

RNA polymerase enzyme catalyzes the transcription process in which RNA is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Summary Introduction

To determine: The similarities and differences of polynucleotide phosphorylase with the above mentioned four enzymes (DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase).

Introduction:

Polynucleotide phosphorylase is a bifunctional enzyme that catalyzes the long chains of polyribonucleotide (RNA). RNA polymerase enzyme catalyzes the transcription process in which RNA polymerase is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

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Answer 4

Question

Chapter 26, Problem 6P

Summary Introduction

To determine: The three properties that are common to the reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase.

Introduction:

RNA polymerase enzyme catalyzes the transcription process in which RNA is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Summary Introduction

To determine: The similarities and differences of polynucleotide phosphorylase with the above mentioned four enzymes (DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase).

Introduction:

Polynucleotide phosphorylase is a bifunctional enzyme that catalyzes the long chains of polyribonucleotide (RNA). RNA polymerase enzyme catalyzes the transcription process in which RNA polymerase is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

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Answer 5

Chapter 26, Problem 6P

Summary Introduction

To determine: The three properties that are common to the reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase.

Introduction:

RNA polymerase enzyme catalyzes the transcription process in which RNA is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Summary Introduction

To determine: The similarities and differences of polynucleotide phosphorylase with the above mentioned four enzymes (DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase).

Introduction:

Polynucleotide phosphorylase is a bifunctional enzyme that catalyzes the long chains of polyribonucleotide (RNA). RNA polymerase enzyme catalyzes the transcription process in which RNA polymerase is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Answer 6

Chapter 26, Problem 6P

Summary Introduction

To determine: The three properties that are common to the reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase.

Introduction:

RNA polymerase enzyme catalyzes the transcription process in which RNA is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Answer 7

Chapter 26, Problem 6P

Summary Introduction

To determine: The three properties that are common to the reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase.

Introduction:

RNA polymerase enzyme catalyzes the transcription process in which RNA is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Answer 8

Summary Introduction

To determine: The similarities and differences of polynucleotide phosphorylase with the above mentioned four enzymes (DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase).

Introduction:

Polynucleotide phosphorylase is a bifunctional enzyme that catalyzes the long chains of polyribonucleotide (RNA). RNA polymerase enzyme catalyzes the transcription process in which RNA polymerase is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Answer 9

Summary Introduction

To determine: The similarities and differences of polynucleotide phosphorylase with the above mentioned four enzymes (DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase).

Introduction:

Polynucleotide phosphorylase is a bifunctional enzyme that catalyzes the long chains of polyribonucleotide (RNA). RNA polymerase enzyme catalyzes the transcription process in which RNA polymerase is synthesized from the DNA template. DNA polymerases are the enzymes that are used for the synthesis of DNA molecule. Reverse transcriptase is the enzyme that is used to produce complementary DNA. RNA replicase helps in the canalization of RNA.

Answer 10

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Answer 13

Ch. 26 - Prob. 1P Ch. 26 - Prob. 2P Ch. 26 - Prob. 3P Ch. 26 - Prob. 4P Ch. 26 - Prob. 5P Ch. 26 - Prob. 6P Ch. 26 - Prob. 7P Ch. 26 - Prob. 8P Ch. 26 - Prob. 9P Ch. 26 - Prob. 10P

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