Principles of Instrumental Analysis
Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Chapter 26, Problem 26.18QAP
Interpretation Introduction

(a)

Interpretation:

Average number of plates should be calculated.

Concept introduction:

Number of theoretical plates is given by following equation:

N = 16(tRW)2

N = number of plates

tR = retention time

W = width of peak base

Average = NA+NB+NC+ND4

Interpretation Introduction

(b)

Interpretation:

Standard deviation for the N should be calculated.

Concept introduction:

Standard deviation will give how the values deviate from mean value of N.

Interpretation Introduction

(c)

Interpretation:

Average plate height of column should be calculated.

Concept introduction:

N=LH

N = plate count

L = length of the packing (cm)

H = height of the column

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4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here.  The elution time for unretained solute is 1.06 min.  The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall.  a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in b
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