When binding of sequence-specific regulatory proteins such as lac repressor or CRP to its respective DNA binding site was studied in vitro, the rates of association were much higher than predicted by collision theory. An explanation for this observation and a simple experimental test of your hypothesis should be explained. Concept Introduction: The lac repressor is a DNA-binding protein that binds to the operator and inhibits the expression of a gene involved in lactose metabolism encoded by lac operon. CRP is a homo-tetramer protein containing two binding subunits composed of two monomers, each containing 360 amino acids. These subunits dimerize forming a tetramer capable of binding to two operator sequences.

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Chapter 26, Problem 23P

Interpretation Introduction

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When binding of sequence-specific regulatory proteins such as lac repressor or CRP to its respective DNA binding site was studied in vitro, the rates of association were much higher than predicted by collision theory. An explanation for this observation and a simple experimental test of your hypothesis should be explained.

Concept Introduction:

The lac repressor is a DNA-binding protein that binds to the operator and inhibits the expression of a gene involved in lactose metabolism encoded by lac operon. CRP is a homo-tetramer protein containing two binding subunits composed of two monomers, each containing 360 amino acids. These subunits dimerize forming a tetramer capable of binding to two operator sequences.

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