The hypothesis that explains the process, by which Botyrococcus braunii contributes to earth’s oil deposits. Introduction: Botryococcus braunii is a pyramid shaped planktonic microalga. This algae is green in color and consists a potentially great importance in the biotecnology. The colonies are found to be present in temperate and tropical oligotropic lakes and estuaries and supported by the lipid bioflim matrix. The presence of elevated levels of dissolved inorganic phosphorus in the water bodies may lead to the blooming of this algae.

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In order to establish the expiration date of perishable food, growth curve data must be collected. Once the microbial load is so high that it poses a hazard to human health, the food item is no longer considered safe (expired). Generally a load of x50,000 bacteria/gram is considered unsafe. Your task is to determine the microbial growth curves for MicroYo, a new brand of yogurt. The growth is determined by sampling the yogurt and growing the bacterial isolates in broth culture which is then serially diluted by a total of x10,000 and inoculated onto standard petri plates of nutrient agar. The following colony counts are measured: Time (days) MicroYo colony count# 1 1 4 1 12 2 16 20 4 7 What day should you recommend expiring the yogurt (the last possible date before the microbial load is unsafe). 12 4 20 16

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Chapter 26, Problem 12TYK

Summary Introduction

To review:

The hypothesis that explains the process, by which Botyrococcus braunii contributes to earth’s oil deposits.

Introduction:

Botryococcus braunii is a pyramid shaped planktonic microalga. This algae is green in color and consists a potentially great importance in the biotecnology. The colonies are found to be present in temperate and tropical oligotropic lakes and estuaries and supported by the lipid bioflim matrix. The presence of elevated levels of dissolved inorganic phosphorus in the water bodies may lead to the blooming of this algae.

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In order to establish the expiration date of perishable food, growth curve data must be collected. Once the microbial load is so high that it poses a hazard to human health, the food item is no longer considered safe (expired). Generally a load of x50,000 bacteria/gram is considered unsafe. Your task is to determine the microbial growth curves for MicroYo, a new brand of yogurt. The growth is determined by sampling the yogurt and growing the bacterial isolates in broth culture which is then serially diluted by a total of x10,000 and inoculated onto standard petri plates of nutrient agar. The following colony counts are measured: Time (days) MicroYo colony count# 1 1 4 1 12 2 16 20 4 7 What day should you recommend expiring the yogurt (the last possible date before the microbial load is unsafe). 12 4 20 16

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