Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 25, Problem 5TQ
Summary Introduction
To review :
The ethical issue to be considered while an attempt is being made to move the acid resistance system from Escherichia coli and Vibrio cholerae.
Introduction:
Vibrio cholerae which is the causative bacteria for cholera disease is a halophilic, comma-shaped, Gram-negative bacteria. This bacterium has pili along with flagellum at one pole. These bacteria are halophile and hence, can tolerate alkaline medium, whichis usually intolerable for intestinal bacteria. However, Vibrio cholerae is sensitive to acid.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 25 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 25.1 - Prob. 1TQCh. 25.4 - Prob. 1TQCh. 25.4 - Prob. 2TQCh. 25.4 - Prob. 3TQCh. 25.4 - Prob. 4TQCh. 25.5 - Prob. 1TQCh. 25.6 - Prob. 1TQCh. 25.6 - Prob. 2TQCh. 25.6 - Prob. 3TQCh. 25.6 - Prob. 4TQ
Ch. 25.7 - Prob. 1TQCh. 25 - Prob. 1RQCh. 25 - Prob. 2RQCh. 25 - Prob. 3RQCh. 25 - Prob. 4RQCh. 25 - Prob. 5RQCh. 25 - Prob. 6RQCh. 25 - Prob. 7RQCh. 25 - Prob. 8RQCh. 25 - Prob. 9RQCh. 25 - Prob. 10RQCh. 25 - Prob. 11RQCh. 25 - Prob. 12RQCh. 25 - Prob. 13RQCh. 25 - Prob. 14RQCh. 25 - Prob. 15RQCh. 25 - Prob. 16RQCh. 25 - Prob. 17RQCh. 25 - Prob. 1TQCh. 25 - Prob. 2TQCh. 25 - Prob. 3TQCh. 25 - Prob. 4TQCh. 25 - Prob. 5TQ
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