Human Anatomy
Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 25, Problem 18TYR
Summary Introduction

To fill:

The correct term in the given blank.

Introduction:

The urinary bladder is a muscular sac like structure found on the floor of the pelvic cavity, posterior to the pubic symphysis andinferior to the peritoneum.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?   2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?   3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1)What is the selectable marker in this experiment? How…

Chapter 25 Solutions

Human Anatomy

Ch. 25.3 - Prob. 9BYGOCh. 25.3 - Prob. 10BYGOCh. 25.3 - Prob. 11BYGOCh. 25.4 - Prob. 12BYGOCh. 25.4 - Prob. 13BYGOCh. 25.4 - Prob. 14BYGOCh. 25.4 - Prob. 15BYGOCh. 25 - Prob. 25.1.1AYLOCh. 25 - Prob. 25.1.2AYLOCh. 25 - Prob. 25.1.3AYLOCh. 25 - To test your knowledge, discuss the following...Ch. 25 - Prob. 25.2.1AYLOCh. 25 - Prob. 25.2.2AYLOCh. 25 - To test your knowledge, discuss the following...Ch. 25 - Prob. 25.2.4AYLOCh. 25 - Prob. 25.2.5AYLOCh. 25 - Prob. 25.2.6AYLOCh. 25 - Prob. 25.2.7AYLOCh. 25 - To test your knowledge, discuss the following...Ch. 25 - To test your knowledge, discuss the following...Ch. 25 - To test your knowledge, discuss the following...Ch. 25 - Prob. 25.2.11AYLOCh. 25 - To test your knowledge, discuss the following...Ch. 25 - Prob. 25.2.13AYLOCh. 25 - To test your knowledge, discuss the following...Ch. 25 - Prob. 25.2.15AYLOCh. 25 - To test your knowledge, discuss the following...Ch. 25 - Prob. 25.3.1AYLOCh. 25 - Prob. 25.3.2AYLOCh. 25 - To test your knowledge, discuss the following...Ch. 25 - Prob. 25.4.1AYLOCh. 25 - Prob. 25.4.2AYLOCh. 25 - Prob. 25.4.3AYLOCh. 25 - Prob. 25.4.4AYLOCh. 25 - Prob. 25.4.5AYLOCh. 25 - To test your knowledge, discuss the following...Ch. 25 - Prob. 25.4.7AYLOCh. 25 - Prob. 1TYRCh. 25 - Prob. 2TYRCh. 25 - Prob. 3TYRCh. 25 - Prob. 4TYRCh. 25 - Prob. 5TYRCh. 25 - A glomerulus and glomerular capsule make up one...Ch. 25 - Prob. 7TYRCh. 25 - Prob. 8TYRCh. 25 - The absorbs variable amounts of water depending on...Ch. 25 - In cortical nophrons, blood of the efferent...Ch. 25 - Prob. 11TYRCh. 25 - Prob. 12TYRCh. 25 - The openings of the two ureters and the urehra...Ch. 25 - Prob. 14TYRCh. 25 - To enter the capsular space, filterate must pass...Ch. 25 - Prob. 16TYRCh. 25 - Prob. 17TYRCh. 25 - Prob. 18TYRCh. 25 - Prob. 19TYRCh. 25 - Prob. 20TYRCh. 25 - Prob. 1BYMVCh. 25 - Prob. 2BYMVCh. 25 - Prob. 3BYMVCh. 25 - Prob. 4BYMVCh. 25 - Prob. 5BYMVCh. 25 - Prob. 6BYMVCh. 25 - Prob. 7BYMVCh. 25 - Prob. 8BYMVCh. 25 - Prob. 9BYMVCh. 25 - Prob. 10BYMVCh. 25 - Prob. 1TOFCh. 25 - Prob. 2TOFCh. 25 - Prob. 3TOFCh. 25 - Prob. 4TOFCh. 25 - Prob. 5TOFCh. 25 - Prob. 6TOFCh. 25 - Prob. 7TOFCh. 25 - Prob. 8TOFCh. 25 - Prob. 9TOFCh. 25 - Prob. 10TOFCh. 25 - Prob. 1TYCCh. 25 - Prob. 2TYCCh. 25 - Prob. 3TYCCh. 25 - Prob. 4TYCCh. 25 - Prob. 5TYC
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