FUNDAMENTALS OF BIOCHEMISTRY - LL FD
5th Edition
ISBN: 9781119598022
Author: Voet
Publisher: WILEY
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Chapter 24, Problem 5E
Summary Introduction
To draw: The structure of cGAMP.
Concept introduction: Cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) is otherwise known as cyclic GMP-AMP. These are the signaling molecules that are produced by the virus infected cells that directly help to initiate the innate immunity in the body, fighting against viruses by restricting the viral replication, and assembly immediately after the infection.
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The preferred substrate is T because the enzyme half-saturates at 7.00 mM for T, but requires 28.0 mM for U, and 112 mM for S.
b
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The rate constant k 2 with substrate S is 9.60×107 sec-1, with substrate T, k 2 = 6.00×104 sec-1, and with substrate U, k 2 = 2.40×106 sec-1.
Calculate the catalytic efficiency with S, T, and U.
Catalytic efficiency with S =
Catalytic efficiency with T =
Catalytic efficiency with U =
Does enzyme A use substrate S, substrate T, or substrate U with greater catalytic efficiency?
Fumerase catalyzes the conversion of fumerate to malate.
fumerate + H2O ⇋ malate
The turnover number, kcat, for fumerase is 8.00×102 sec-1. The Km of this enzyme for fumerate is 5.00×10-3 μmol mL-1.
a
In an experiment using 2.00×10-3 μmol·mL-1, what is Vmax?
Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and Na2HPO4. If the final solution was 0.18 M in KH2PO4, you would need 0.25 M Na2HPO4. Now assume you wish to make a buffer at the same pH, using the same substances, but want the total phosphate molarity ([HPO42−]+[H2PO−4]) to equal 0.20 M. What concentration of the Na2HPO4 would be required?
Chapter 24 Solutions
FUNDAMENTALS OF BIOCHEMISTRY - LL FD
Ch. 24 - Prob. 1ECh. 24 - Prob. 2ECh. 24 - 3. Amino acid residues in proteins are each...Ch. 24 - Prob. 4ECh. 24 - Prob. 5ECh. 24 - Prob. 6ECh. 24 - Prob. 7ECh. 24 - 8. Show schematically how a single strand of four...Ch. 24 - Prob. 9ECh. 24 - Prob. 10E
Ch. 24 - Prob. 11ECh. 24 - Prob. 12ECh. 24 - Prob. 13ECh. 24 - Prob. 14ECh. 24 - Prob. 15ECh. 24 - Prob. 16ECh. 24 - Prob. 17ECh. 24 - Prob. 18ECh. 24 - 19. What is the probability that the palindromic...Ch. 24 - Prob. 20ECh. 24 - Prob. 21ECh. 24 - Prob. 22ECh. 24 - Prob. 23ECh. 24 - Prob. 24ECh. 24 - Prob. 25ECh. 24 - 26. Calculate the length of the 50,000-bp DNA in a...Ch. 24 - Prob. 27ECh. 24 - Prob. 28ECh. 24 - Prob. 29CQCh. 24 - Prob. 30CQCh. 24 - Prob. 31CQCh. 24 - Prob. 32CQCh. 24 - Prob. 33CQCh. 24 - Prob. 34CQCh. 24 - Prob. 35CQCh. 24 - Prob. 36CQCh. 24 - Prob. 37CQCh. 24 - Prob. 38CQCh. 24 - Prob. 1MTE
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