Chapter 24, Problem 24.27P

a)

Interpretation Introduction

Interpretation:

The response factor for 2, 3-dimethyl-2-butanol has to be calculated.

Concept Introduction:

Quantitative analysis with internal standard can be given by the formula of,

$\frac{{\text{A}}_{\text{x}}}{\left[\text{X}\right]}\text{=F}\frac{{\text{A}}_{\text{s}}}{\left[\text{S}\right]}$

Here ${\text{A}}_{\text{x}}$ = area of analyte signal

${\text{A}}_{\text{s}}$ = area of internal standard

$\left[\text{X}\right]$ = analyte’s concentration

$\left[\text{S}\right]$ = concentration of internal standard

$\text{F}$ = response factor

To calculate the response factor for 2, 3-dimethyl-2-butanol

b)

Interpretation Introduction

Interpretation:

The areas of Pentanol and 2, 3-dimethyl-2-butanol has to be calculated.

Concept Introduction:

Area of peaks:

The area of Gaussian peak can be calculated by the formula below,

Area of Gaussian peak = $\text{1}\text{.064×peak}{\text{height×w}}_{\text{1/2}}$

Here ${\text{w}}_{\text{1/2}}$ = width at half-height

To calculate the areas of Pentanol and 2,3-dimethyl-2-butanol

c)

Interpretation Introduction

Interpretation:

The concentration of 2,3-dimethyl-2-butanol has to be calculated.

Concept Introduction:

Quantitative analysis with internal standard can be given by the formula of,

$\frac{{\text{A}}_{\text{x}}}{\left[\text{X}\right]}\text{=F}\frac{{\text{A}}_{\text{s}}}{\left[\text{S}\right]}$

Here ${\text{A}}_{\text{x}}$ = area of analyte signal

${\text{A}}_{\text{s}}$ = area of internal standard

$\left[\text{X}\right]$ = analyte’s concentration

$\left[\text{S}\right]$ = concentration of internal standard

$\text{F}$ = response factor

To calculate the concentration of 2, 3-dimethyl-2-butanol