Chapter 24, Problem 24.30P

a)

Interpretation Introduction

Interpretation:

The reason why diluted tequila is extracted by four times with dichloromethane rather than one time with a higher volume has to be explained.

To explain the reason why diluted tequila is extracted by four times with dichloromethane rather than one time with a higher volume

b)

Interpretation Introduction

Interpretation:

The reason for the usage of on-column injection has to be explained.

Concept Introduction:

On-Column injection:

On-column injection is the best method for quantitative analysis for compounds that are thermally sensitive. It is low resolution technique and doesn’t support for columns with diameters less than 0.2 mm. On-column injection can be used to handle solutions that dilute and concentration in solution both in large and small volumes.

To explain the usage of on-column injection

c)

Interpretation Introduction

Interpretation:

The reason for the usage of poly (ethylene glycol) column has to be explained.

To explain the usage of poly (ethylene glycol) column

d)

Interpretation Introduction

Interpretation:

The phase ratio $\text{β}$ has to be calculated.

Concept Introduction:

Phase ratio $\beta$:

The volume of mobile phase divided by the volume of the stationary phase is called as dimensionless phase ratio. The phase ratio is calculated as,

$\text{β=}\frac{\text{r}}{{\text{2d}}_{\text{f}}}$

Where, $\text{β}$ = phase ratio

$\text{r}$ = radius of column

${\text{d}}_{\text{f}}$ = thickness of stationary phase time

Increase in thickness of stationary phase, decreases in $\text{β}$, that increases the retention time and capacity of sample.

To calculate the phase ratio $\text{β}$

e)

Interpretation Introduction

Interpretation:

The reason for the use of $\text{0}\text{.53}\text{mm}$ diameter of wide-bore column has to be explained.

To explain the reason for the use of $\text{0}\text{.53}\text{mm}$ diameter of wide-bore column