Chapter 24, Problem 24.24P

a)

Interpretation Introduction

Interpretation:

The reason has to be given why it’s not good to use a thin stationary phase ( $\text{0}\text{.2μm}$) in wide bore ( $\text{0}\text{.53}\text{mm}$) open tubular column.

Concept Introduction:

Open tubular columns:

The open tubular columns are made of silica (in fused form) and is coated with Polyimide for the support and to protect from atmospheric moisture. The most common type of open tubular column is wall coated column and the less common type is porous layer column. The columns are coiled in such way that they fit within a particular temperature controlled column oven.

Advantages and disadvantages of open tubular columns:

Open tubular columns provides higher resolution but short time analysis and show greater sensitivity when compared to packed columns. These columns have less capacity of the sample.

Deemter equation:

The Deemter equation is given as,

$\text{H}\approx \text{A+}\frac{\text{B}}{{\text{U}}_{\text{x}}}{\text{+Cu}}_{\text{x}}$

Where A is multiple paths

$\frac{\text{B}}{{\text{U}}_{\text{x}}}$ is Longitudinal diffusion

${\text{Cu}}_{\text{x}}$ is equilibration time

$\text{H}$ is height of plate

To explain the reason why it’s not good to use a thin stationary phase ( $\text{0}\text{.2μm}$) in wide bore ( $\text{0}\text{.53}\text{mm}$) open tubular column

b)

Interpretation Introduction

Interpretation:

The mass of stationary phase in each column and the number of grams (in nanograms) of analyte that can be injected into each column has to be calculated.

To calculate the mass of stationary phase in each column and the number of grams (in nanograms) of analyte that can be injected into each column