(a) Interpretation: The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined. Concept introduction: Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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Answer 1

Question

Chapter 23, Problem 46P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 23, Problem 46P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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Answer 3

Question

Chapter 23, Problem 46P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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Answer 4

Question

Chapter 23, Problem 46P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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Answer 5

Chapter 23, Problem 46P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 6

Chapter 23, Problem 46P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 7

Chapter 23, Problem 46P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 12

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 13

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 14

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 15

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 16

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 17

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 18

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 19

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 20

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Answer 21

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Answer 22

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Answer 23

Ch. 23 - Prob. 1P Ch. 23 - Prob. 2P Ch. 23 - Prob. 3P Ch. 23 - Prob. 4P Ch. 23 - Prob. 5P Ch. 23 - Prob. 6P Ch. 23 - Prob. 7P Ch. 23 - Prob. 8P Ch. 23 - Prob. 9P Ch. 23 - Prob. 10P

Solution Summary: The author analyzes the effect of PAN on archaeal proteasome activity in the absence of nucleotides.

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