The percentage of deaths caused by A. ostoyae in the most affected species in both control and experimental forests. Introduction: Parasitic fungi attack plants and withdraw nutrients from them. Armillaria ostoyae (honey mushroom) is an example of fungal parasite . They attack the root systems of living trees. When the tree dies, the fungal parasite survives on the dead remains. The hyphae that grow from roots of infected trees and dead stumps invade roots of healthy plants leading to infection.

Question

a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________

Answer 1

Question

Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 23, Problem 2DAA

Summary Introduction

To determine: The percentage of deaths caused by A. ostoyae in the most affected species in both control and experimental forests.

Introduction: Parasitic fungi attack plants and withdraw nutrients from them. Armillaria ostoyae (honey mushroom) is an example of fungal parasite. They attack the root systems of living trees. When the tree dies, the fungal parasite survives on the dead remains. The hyphae that grow from roots of infected trees and dead stumps invade roots of healthy plants leading to infection.

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a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________

1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?

1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Answer 2

Question

Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 23, Problem 2DAA

Summary Introduction

To determine: The percentage of deaths caused by A. ostoyae in the most affected species in both control and experimental forests.

Introduction: Parasitic fungi attack plants and withdraw nutrients from them. Armillaria ostoyae (honey mushroom) is an example of fungal parasite. They attack the root systems of living trees. When the tree dies, the fungal parasite survives on the dead remains. The hyphae that grow from roots of infected trees and dead stumps invade roots of healthy plants leading to infection.

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Answer 3

Question

Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 23, Problem 2DAA

Summary Introduction

To determine: The percentage of deaths caused by A. ostoyae in the most affected species in both control and experimental forests.

Introduction: Parasitic fungi attack plants and withdraw nutrients from them. Armillaria ostoyae (honey mushroom) is an example of fungal parasite. They attack the root systems of living trees. When the tree dies, the fungal parasite survives on the dead remains. The hyphae that grow from roots of infected trees and dead stumps invade roots of healthy plants leading to infection.

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Answer 4

Question

Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 23, Problem 2DAA

Summary Introduction