Chapter 23, Problem 23.21P

Interpretation Introduction

Interpretation:

$\log \left(\text{Molecular}\text{weight}\right)$ versus $\frac{\text{1}}{\text{relative migration}\text{time}}$ has to be plotted. Molecular mass of ferritin light and heavy chains has to be plotted.

Concept Introduction:

The logarithm of molecular mass of the SDS-coated protein is proportional to $\frac{\text{1}}{\text{relative}\text{retention}\text{time}}$ of the protein through the gel. Absolute migration times are somewhat variable from run to run, so relative migration times are measured. The relative migration time is the migration time of a protein divided by the migration time of a fast-moving small dye molecule.