Chapter 23, Problem 23.18P

(a)

Interpretation Introduction

Interpretation:

Principal source of band broadening in ideal capillary zone electrophoresis has to be explained. Van Deemter curve for capillary zone electrophoresis has to be plotted.

Concept Introduction:

Plate height of chromatogram can be calculated by van Deemter equation as follows:

    $\text{H}\approx \text{A}+\frac{\text{B}}{\text{u}}+\left(\text{C}\right)\left(\text{u}\right)$

Here $\text{H}$ is the plate height.

$\text{A}$ term is the eddy diffusion constant.

$\text{B}$ term is the longitudinal diffusion constant.

$\text{C}$ term is the mass transfer constant.

$\text{u}$ is the migration velocity.

The term $\text{A}$ that is the eddy diffusion constant is related to the particle size of the material and consistency of the flow around the particles but it is independent of the flow rate.

The term $\text{B}$ that is the longitudinal diffusion is related to the diffusion of the analyte in mobile phase and the stationary phase.

The term $\text{C}$ that is the mass transfer constant that is related to the flow rate and the square of the particle size.

(b)

Interpretation Introduction

Interpretation:

Source of band broadening in micellar electrokinetic capillary chromatography has to be explained. Van Deemter curve for micellar electrokinetic capillary chromatography has to be drawn.

Concept Introduction:

Refer to part (a).