EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 22, Problem 6MC
Summary Introduction
Introduction:
Group A Streptococcus is the cause of scarlet fever, rheumatic fever, and bacterial pharyngitis. This survives in the body using several factors, including M protein, pyrogenic toxins, a hyaluronic acid, streptolysin, and enzymes. These allow the pathogen to spread in many different ways in the human body.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 22 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 22 - Prob. 1TMWCh. 22 - Why must diphtheria immunization be boosted every...Ch. 22 - Why is it inappropriate to treat a cold with...Ch. 22 - Prob. 1CCSCh. 22 - Prob. 4TMWCh. 22 - Prob. 1EDCSCh. 22 - Prob. 2CCSCh. 22 - Prob. 5TMWCh. 22 - Prob. 2EDCSCh. 22 - Outbreaks of blastomycosis have occurred in Latin...
Ch. 22 - Prob. 1MCCh. 22 - Prob. 2MCCh. 22 - Prob. 3MCCh. 22 - The glycoprotein spikes on influenzaviruses are...Ch. 22 - Prob. 5MCCh. 22 - Prob. 6MCCh. 22 - Prob. 7MCCh. 22 - Prob. 8MCCh. 22 - Prob. 9MCCh. 22 - Prob. 10MCCh. 22 - Prob. 1FIBCh. 22 - Prob. 2FIBCh. 22 - Prob. 3FIBCh. 22 - Fill in the Blanks 4. A drug commonly used to...Ch. 22 - Prob. 5FIBCh. 22 - Prob. 1MTFCh. 22 - Prob. 2MTFCh. 22 - Prob. 3MTFCh. 22 - Prob. 4MTFCh. 22 - Prob. 5MTFCh. 22 - Prob. 1VICh. 22 - Identify the following bacteria discussed in this...Ch. 22 - Prob. 1SACh. 22 - Prob. 2SACh. 22 - Prob. 3SACh. 22 - After listening to a lecture on diseases caused by...Ch. 22 - Prob. 5SACh. 22 - Prob. 6SACh. 22 - Prob. 7SACh. 22 - Prob. 8SACh. 22 - Prob. 9SACh. 22 - Prob. 10SACh. 22 - Prob. 1CTCh. 22 - An elderly man is admitted to the hospital with...Ch. 22 - Compare and contrast viral pneumonia with...Ch. 22 - Prob. 4CTCh. 22 - Prob. 5CTCh. 22 - Compare and contrast antigenic drift and antigenic...Ch. 22 - In mid-November, a worried couple brought their...Ch. 22 - Prob. 8CTCh. 22 - Prob. 9CTCh. 22 - Prob. 10CTCh. 22 - Prob. 11CTCh. 22 - Prob. 1CM
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