EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 22, Problem 5CT
Summary Introduction
To answer:
A patient is admitted to a hospital in Ohio with a respiratory fungal infection. He had returned the previous month from a trip to Arizona, where he bought some dusty old blankets and two pots from a roadside vendor. What diseases might he have.
Introduction:
Coccidioidomycosis is also known as California fever. It is infected by coccidiosis immitis, mostly occurs in California and it is an air borne disease. Chest pain is there and difficulty in breathing this can be cured by Amphotericin B.
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Overview of Transformation Protocol
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Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
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Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
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Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 22 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 22 - Prob. 1TMWCh. 22 - Why must diphtheria immunization be boosted every...Ch. 22 - Why is it inappropriate to treat a cold with...Ch. 22 - Prob. 1CCSCh. 22 - Prob. 4TMWCh. 22 - Prob. 1EDCSCh. 22 - Prob. 2CCSCh. 22 - Prob. 5TMWCh. 22 - Prob. 2EDCSCh. 22 - Outbreaks of blastomycosis have occurred in Latin...
Ch. 22 - Prob. 1MCCh. 22 - Prob. 2MCCh. 22 - Prob. 3MCCh. 22 - The glycoprotein spikes on influenzaviruses are...Ch. 22 - Prob. 5MCCh. 22 - Prob. 6MCCh. 22 - Prob. 7MCCh. 22 - Prob. 8MCCh. 22 - Prob. 9MCCh. 22 - Prob. 10MCCh. 22 - Prob. 1FIBCh. 22 - Prob. 2FIBCh. 22 - Prob. 3FIBCh. 22 - Fill in the Blanks 4. A drug commonly used to...Ch. 22 - Prob. 5FIBCh. 22 - Prob. 1MTFCh. 22 - Prob. 2MTFCh. 22 - Prob. 3MTFCh. 22 - Prob. 4MTFCh. 22 - Prob. 5MTFCh. 22 - Prob. 1VICh. 22 - Identify the following bacteria discussed in this...Ch. 22 - Prob. 1SACh. 22 - Prob. 2SACh. 22 - Prob. 3SACh. 22 - After listening to a lecture on diseases caused by...Ch. 22 - Prob. 5SACh. 22 - Prob. 6SACh. 22 - Prob. 7SACh. 22 - Prob. 8SACh. 22 - Prob. 9SACh. 22 - Prob. 10SACh. 22 - Prob. 1CTCh. 22 - An elderly man is admitted to the hospital with...Ch. 22 - Compare and contrast viral pneumonia with...Ch. 22 - Prob. 4CTCh. 22 - Prob. 5CTCh. 22 - Compare and contrast antigenic drift and antigenic...Ch. 22 - In mid-November, a worried couple brought their...Ch. 22 - Prob. 8CTCh. 22 - Prob. 9CTCh. 22 - Prob. 10CTCh. 22 - Prob. 11CTCh. 22 - Prob. 1CM
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