(a) Interpretation: The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined. The K M and V max values of wild-type and mutant enzymes must be determined. Concept introduction: Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

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Answer 1

Question

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

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Answer 2

Question

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

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Answer 3

Question

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

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Answer 4

Question

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

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Answer 5

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 6

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 7

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 12

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 13

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 14

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 15

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 16

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Answer 17

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Answer 18

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Answer 19

Ch. 22 - Prob. 1P Ch. 22 - Prob. 2P Ch. 22 - Prob. 3P Ch. 22 - Prob. 4P Ch. 22 - Prob. 5P Ch. 22 - Prob. 6P Ch. 22 - Prob. 7P Ch. 22 - Prob. 8P Ch. 22 - Prob. 9P Ch. 22 - Prob. 10P

Solution Summary: The author explains that the effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

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Chapter 22 Solutions