Hole's Essentials of Human Anatomy & Physiology
Hole's Essentials of Human Anatomy & Physiology
13th Edition
ISBN: 9781259277368
Author: David N. Shier Dr., Jackie L. Butler, Ricki Lewis Dr.
Publisher: McGraw-Hill Education
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Chapter 2.2, Problem 13P
Summary Introduction

To define: Buffer.

Introduction: The pH can be defined as the concentration of hydrogen ion in a solution. It ranges from 0 to 14. The human blood’s pH is roughly 7.4.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin

Chapter 2 Solutions

Hole's Essentials of Human Anatomy & Physiology

Ch. 2.2 - Prob. 3PCh. 2.2 - Prob. 4PCh. 2.2 - What is an ion? Ch. 2.2 - Prob. 6PCh. 2.2 - Prob. 7PCh. 2.2 - Prob. 8PCh. 2.2 - How does the hydrogen ion concentration compare...Ch. 2.2 - What is a molecular formula? A structural...Ch. 2.2 - Describe three kinds of chemical reactions. Ch. 2.2 - Compare the characteristics of an acid with those...Ch. 2.2 - What does pH measure? Ch. 2.2 - Prob. 13PCh. 2.3 - List the major inorganic chemicals in cells and...Ch. 2.3 - Describe the general functions of the four main...Ch. 2.3 - Prob. 14PCh. 2.3 - How do electrolytes and nonelectrolytes differ? Ch. 2.3 - Name the inorganic substances common in body...Ch. 2.3 - Why is it incorrect to say that “fat” is another...Ch. 2.3 - Compare the chemical composition of carbohydrates,...Ch. 2.3 - How does an enzyme affect a chemical reaction? Ch. 2.3 - What is the chemical basis of the great diversity...Ch. 2.3 - What are the functions of nucleic acids? Ch. 2 - Prob. 1CACh. 2 - Prob. 2CACh. 2 - Prob. 3CACh. 2 - Prob. 4CACh. 2 - Prob. 5CACh. 2 - Prob. 6CACh. 2 - Prob. 7CACh. 2 - Prob. 8CACh. 2 - Prob. 9CACh. 2 - Prob. 10CACh. 2 - Prob. 11CACh. 2 - Prob. 12CACh. 2 - Prob. 13CACh. 2 - Prob. 14CACh. 2 - Prob. 15CACh. 2 - Prob. 16CACh. 2 - Prob. 17CACh. 2 - Prob. 18CACh. 2 - Prob. 19CACh. 2 - Distinguish between electrolytes and...Ch. 2 - Prob. 21CACh. 2 - List several ions in body fluids. Ch. 2 - Describe the general characteristics of...Ch. 2 - Distinguish between simple sugars and complex...Ch. 2 - Describe the general characteristics of lipids,...Ch. 2 - A triglyceride molecule consists of __________. 3...Ch. 2 - Explain the difference between saturated and...Ch. 2 - Prob. 28CACh. 2 - List at least three functions of proteins. Ch. 2 - Describe four levels of protein structure. Ch. 2 - Prob. 31CACh. 2 - Describe the structure of nucleic acids. Ch. 2 - Explain the major functions of nucleic acids. Ch. 2 - Prob. 1IACTCh. 2 - Prob. 2IACTCh. 2 - Prob. 3IACTCh. 2 - A topping for ice cream contains fructose,...Ch. 2 - Prob. 5IACTCh. 2 - Prob. 6IACTCh. 2 - Prob. 7IACT
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